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AB326905

Mouse FSTL1/FRP ELISA Kit, Chemiluminescent

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Mouse FSTL1/FRP ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse FSTL1/FRP with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.01 - 30 ng/ml with a sensitivity of 0.01 ng/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Frp, Fstl, Tsc36, Fstl1, Follistatin-related protein 1, Follistatin-like protein 1, TGF-beta-inducible protein TSC-36

3 Images
Sandwich ELISA - Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (AB326905)
  • sELISA

Lab

Sandwich ELISA - Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (AB326905)

Standard curve comparison between the Chemiluminescent Mouse FSTL1/FRP SimpleStep ELISA and colorimetric Mouse FSTL1/FRP SimpleStep ELISA (ab316895) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (AB326905)
  • sELISA

Lab

Sandwich ELISA - Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (AB326905)

Dynamic range comparison between the Chemiluminescent Mouse FSTL1/FRP SimpleStep ELISA, colorimetric Mouse FSTL1/FRP SimpleStep ELISA (ab316895), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (AB326905)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (AB326905)

Interpolated concentrations of mouse FSTL1/FRP in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native FSTL1/FRP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 4%, plasma (citrate) 10%, plasma (EDTA) 12%, and plasma (heparin) 5%. The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Mouse, Rat

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.01 ng/mL

Range

0.01 - 30 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (ab326905) is a rapid single-wash sandwich 55-min ELISA to measure Mouse FSTL1/FRP in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media with a sensitivity of 0.01 ng/mL.

How the assay works

Mouse FSTL1/FRP ELISA Kit, Chemiluminescent (ab326905) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse FSTL1/FRP (ab326905) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Follistatin related protein 1 (FSTL1) antibody [EPR23972-46] - BSA and Aside free (Capture) ab281160
- Anti-Follistatin related protein 1 (FSTL1) antibody [EPR23972-85] - BSA and Aside free (Detector) ab281010
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2" } ]
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Recovery

[ { "sample": "Serum", "range": "105 - 122 %", "average": "= 122" }, { "sample": "Citrate plasma", "range": "80 - 84 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "98 - 106 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "106 - 131 %", "average": "= 131" }, { "sample": "Cell culture media", "range": "109 - 121 %", "average": "= 121" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (By similarity). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (PubMed : 19595790, PubMed : 21826198). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (PubMed : 20054002). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (By similarity).
See full target information Fstl1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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