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AB272776

Mouse FXII ELISA kit (total FXII antigen)

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Mouse FXII ELISA kit (total FXII antigen) is a sandwich ELISA designed to quantify total mouse FXII antigen with a sensitivity of 0.005 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.01 - 10 ng/mL

View Alternative Names

Coagulation factor XII, Hageman factor, HAF, F12

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ELISA - Mouse FXII ELISA kit (total FXII antigen) (AB272776)
  • ELISA

Supplier Data

ELISA - Mouse FXII ELISA kit (total FXII antigen) (AB272776)

A typical standard curve. Example only.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, EDTA Plasma

Reacts with

Mouse

Results type

Quantitative

Range

0.01 - 10 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse FXII ELISA kit (total FXII antigen) (ab272776) is intended for the quantitative determination of total Factor XII antigen in mouse plasma.

Mouse Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti‑mouse Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse Factor XII. Color development is proportional to the concentration of Factor XII in the samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 10, "mean": "0.238 ng/mL", "standardDeviation": null, "coefficientOfVariability": "9.04" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 10, "mean": "0.725 ng/mL", "standardDeviation": null, "coefficientOfVariability": "5.52" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 10, "mean": "2.04 ng/mL", "standardDeviation": null, "coefficientOfVariability": "6.19" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 20, "mean": "0.055 ng/mL", "standardDeviation": null, "coefficientOfVariability": "6.25" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 20, "mean": "0.29 ng/mL", "standardDeviation": null, "coefficientOfVariability": "2.88" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 20, "mean": "0.825 ng/mL", "standardDeviation": null, "coefficientOfVariability": "1.71" } ]
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Recovery

[ { "sample": "Spike", "range": "94 - 102 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272776-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"10X Wash Buffer", "number":"AB272776-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"FXII ELISA Plate", "number":"AB272776-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Anti-Mouse FXII Primary Antibody Lyophilized Vial", "number":"AB272776-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse FXII Standard Lyophilized Vial", "number":"AB272776-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Streptavidin-HRP Secondary Reagent", "number":"AB272776-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB272776-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor XII also known as FXII or Hageman factor is a serine protease and a coagulation factor that plays an important role in initiating the intrinsic pathway of blood coagulation. It has a molecular mass of approximately 80 kDa. The liver primarily synthesizes this protein and it circulates in the blood plasma as an inactive precursor. When activated to Factor XIIa it cleaves and activates other coagulation factors such as Factor XI to propagate the coagulation cascade that helps in blood clot formation.
Biological function summary

This protein helps control blood coagulation processes as part of a larger network of interacting proteins that includes contact activation factors. Factor XII activates not only the coagulation cascade but also pathways involved in fibrinolysis inflammation and bradykinin formation. It triggers the kallikrein-kinin system and leads to the generation of bradykinin a peptide known to induce vascular permeability and inflammation.

Pathways

The involvement of Factor XII spans the intrinsic coagulation pathway and the kallikrein-kinin system. It interacts with several other coagulation factors such as Factor XI and prekallikrein to advance the clotting process. The protein initiates the intrinsic blood coagulation pathway by converting Factor XI to Factor XIa in the presence of negatively charged surfaces. It links to the fibrinolytic pathway and can indirectly activate plasminogen through interactions that generate plasmin.

Factor XII deficiency leads to increased risk of thrombosis even though such deficiency generally shows no major bleeding symptoms. The target links to hereditary angioedema where dysregulation in the kallikrein-kinin system involves premature bradykinin activity. Factor XII associates with C1-inhibitor a protein playing a regulatory role in preventing excessive bradykinin production which can manifest in clinical symptoms seen in hereditary angioedema patients.
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Product protocols

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Target data

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (By similarity).
See full target information Coagulation factor XII
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websiteProtocolBooklet
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Product promise

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