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AB100684

Mouse G-CSF ELISA Kit (CSF3)

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Mouse G-CSF ELISA Kit (CSF3) is a Sandwich (quantitative) ELISA for the measurement of Mouse G-CSF (CSF3) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF

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Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB100684)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB100684)

Representative Standard Curve using ab100684

Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB100684)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB100684)

Representative Standard Curve using ab100684

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 0.5 pg/mL

Range

0.62 - 150 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's G-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse G-CSF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse G-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 97.52" }, { "sample": "Serum", "range": "83 - 104 %", "average": "= 95.53" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 94.28" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100684-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100684-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100684-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"G-CSF Microplate (12 x 8 wells)", "number":"AB100684-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100684-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100684-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse G-CSF Standard (lyophilized)", "number":"AB100684-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"80x HRP-Streptavidin Concentrate", "number":"AB100684-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse G-CSF", "number":"AB100684-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100684-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
See full target information Csf3
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websiteProtocolBooklet
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Product promise

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