Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min Simplestep used to quantify Mouse G-CSF (CSF3) with a sensitivity of 5.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Frontiers in immunology 14:11322652023Bioactive compound C498-0670 alleviates LPS-induced sepsis JAK/STAT and NFκB signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Xu,Xinxin Zhang,Mingming Zhou,Peizhe Lu,Yuting Xu,Lihong Wu,Qianyue Zhang,Zhihua Wu,Xiaoyu Xu,Pengfei Shi,Qingda Wei,Xiaoyu Li,Qiaoling SongPubMed 37122731
- Frontiers in immunology 12:6412062021Receptor Tyrosine Kinase Inhibitor Sunitinib as Novel Immunotherapy to Inhibit Myeloid-Derived Suppressor Cells for Treatment of Endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing He,Sze Wan Hung,Bo Liang,Ruizhe Zhang,Yating Gao,Ching Yan Chu,Tao Zhang,Hui Xu,Jacqueline Pui Wah Chung,Chi Chiu WangPubMed 34367125
- Journal of cellular and molecular medicine 25:6333-63472021Scaffold compound L971 exhibits anti-inflammatory activities through inhibition of JAK/STAT and NFκB signalling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyuan Li,Yu Yan,Xinxin Zhang,Yidan Zhang,Xiaohan Xu,Lei Zhang,Liangliang Lu,Jie Wang,Yazhuo Zhang,Qiaoling Song,Chenyang ZhaoPubMed 34018320
- Cell communication and signaling : CCS 18:522020Exosomal KRAS mutation promotes the formation of tumor-associated neutrophil extracellular traps and causes deterioration of colorectal cancer by inducing IL-8 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnquan Shang et. al.PubMed 32228650
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Alternative names
Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF
What's included?
Recommended products
Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min Simplestep used to quantify Mouse G-CSF (CSF3) with a sensitivity of 5.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 5.4 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample RAW 264.7 | n 5 | C.V. 5.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample RAW 264.7 | n 3 | C.V. 11.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97.2 | Range 86.2 - 106 % |
Sample type Citrate plasma | Average % = 105.2 | Range 95.1 - 116.9 % |
Sample type Cell culture media | Average % = 103.5 | Range 98 - 106.5 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse G-CSF ELISA Kit (CSF3) (ab197743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF (CSF3) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse G-CSF (CSF3) with 5.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.
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5 product images
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)
Example of G-CSF standard curve for Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)
Example of G-CSF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)
Interpolated concentrations of native G-CSF in mouse serum and plasma (citrate) samples.
The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)
Interpolated concentrations of native G-CSF in RAW264.7 LPSstimulated cell culture supernatant samples.
RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)
LPS-treatment of RAW 264.7 cells stimulates secretion of G-CSF.
RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. Various dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of G-CSF adjusted for sample dilution are graphed in pg of G-CSF per mL of supernatant (mean +/- SD, n = 2). Note that G-CSF is not detectable in untreated RAW 264.7 supernatant samples.
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