Mouse G-CSF ELISA Kit (CSF3)
- Recombinant
- SimpleStep
- What is this?
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(6 Publications)
Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min Simplestep used to quantify Mouse G-CSF (CSF3) with a sensitivity of 5.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
View Alternative Names
Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF
- ELISA
Supplier Data
ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB197743)
- sELISA
Unknown
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB197743)
Interpolated concentrations of native G-CSF in RAW264.7 LPS stimulated cell culture supernatant samples.
RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : RAW264.7 supernatant 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB197743)
Interpolated concentrations of native G-CSF in mouse serum and plasma (citrate) samples.
The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB197743)
Example of G-CSF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB197743)
LPS-treatment of RAW 264.7 cells stimulates secretion of G-CSF.
RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. Various dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of G-CSF adjusted for sample dilution are graphed in pg of G-CSF per mL of supernatant (mean +/- SD, n = 2). Note that G-CSF is not detectable in untreated RAW 264.7 supernatant samples.
- sELISA
Supplier Data
Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (AB197743)
Example of G-CSF standard curve for Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Mouse G-CSF ELISA Kit (CSF3) ab197743 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse G-CSF (CSF3)in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 5.4 pg/mL.
How the assay works
Mouse G-CSF (CSF3)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse G-CSF ELISA Kit (CSF3) ab197743 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (6)
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International journal of molecular sciences 26: PubMed40429945
2025
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Journal of orthopaedic surgery and research 19:517 PubMed39198853
2024
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Frontiers in immunology 14:1132265 PubMed37122731
2023
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Frontiers in immunology 12:641206 PubMed34367125
2021
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Journal of cellular and molecular medicine 25:6333-6347 PubMed34018320
2021
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Cell communication and signaling : CCS 18:52 PubMed32228650
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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