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Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse G-CSF (CSF3) in Citrate plasma, Cell culture supernatant, Serum samples.

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Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Sensitivity

= 5.4 pg/mL

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Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

What's included?

1 x 96 Tests
Components
10X Mouse G-CSF Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Mouse G-CSF Capture Antibody (Lyophilized)
1 x 1 Vial
Mouse G-CSF Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent 25BS
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse G-CSF (CSF3) in Citrate plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 5.4 pg/mL

Precision

Intra assay

Sample
RAW 264.7
n
5
mean
-
SD
-
C.V.
5.7

Inter assay

Sample
RAW 264.7
n
3
mean
-
SD
-
C.V.
11.9

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 97.2
Range
86.2 - 106 %
Sample type
Citrate plasma
Average %
= 105.2
Range
95.1 - 116.9 %
Sample type
Cell culture media
Average %
= 103.5
Range
98 - 106.5 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse G-CSF ELISA Kit (CSF3) (ab197743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF (CSF3) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse G-CSF (CSF3) with 5.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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