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Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse G-CSF (CSF3) in Citrate plasma, Cell culture supernatant, Serum samples.

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Images

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Sensitivity

= 5.4 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Alternative names

What's included?

1 x 96 Tests
Components
10X Mouse G-CSF Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Mouse G-CSF Capture Antibody (Lyophilized)
1 x 1 Vial
Mouse G-CSF Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent 25BS
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse G-CSF (CSF3) in Citrate plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 5.4 pg/mL

Precision

Intra assay

Sample

RAW 264.7

n

5

mean

-

SD

-

C.V.

5.7

Inter assay

Sample

RAW 264.7

n

3

mean

-

SD

-

C.V.

11.9

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 97.2

Range

86.2 - 106 %

Sample type

Citrate plasma

Average %

= 105.2

Range

95.1 - 116.9 %

Sample type

Cell culture media

Average %

= 103.5

Range

98 - 106.5 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse G-CSF ELISA Kit (CSF3) (ab197743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF (CSF3) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse G-CSF (CSF3) with 5.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743), expandable thumbnail

    Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)

    Example of G-CSF standard curve for Sample Diluent 25BS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743), expandable thumbnail

    Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)

    Example of G-CSF standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743), expandable thumbnail

    Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)

    Interpolated concentrations of native G-CSF in mouse serum and plasma (citrate) samples.

    The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743), expandable thumbnail

    Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)

    Interpolated concentrations of native G-CSF in RAW264.7 LPSstimulated cell culture supernatant samples.

    RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743), expandable thumbnail

    Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)

    LPS-treatment of RAW 264.7 cells stimulates secretion of G-CSF.

    RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. Various dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of G-CSF adjusted for sample dilution are graphed in pg of G-CSF per mL of supernatant (mean +/- SD, n = 2). Note that G-CSF is not detectable in untreated RAW 264.7 supernatant samples.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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