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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse G-CSF (CSF3) in Citrate plasma, Cell culture supernatant, Serum samples.
Colorimetric
Citrate plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Mouse
12.5 - 800 pg/mL
1h 30m
= 5.4 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Granulocyte colony-stimulating factor, G-CSF, Csfg, Csf3
Mouse G-CSF ELISA Kit (CSF3) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse G-CSF (CSF3) in Citrate plasma, Cell culture supernatant, Serum samples.
Granulocyte colony-stimulating factor, G-CSF, Csfg, Csf3
Colorimetric
Citrate plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Mouse
12.5 - 800 pg/mL
1h 30m
Microplate
= 5.4 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample RAW 264.7 | n 5 | mean - | SD - | C.V. 5.7 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample RAW 264.7 | n 3 | mean - | SD - | C.V. 11.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 97.2 | Range 86.2 - 106 % |
Sample type Citrate plasma | Average % = 105.2 | Range 95.1 - 116.9 % |
Sample type Cell culture media | Average % = 103.5 | Range 98 - 106.5 % |
Blue Ice
+4°C
+4°C
Mouse G-CSF ELISA Kit (CSF3) (ab197743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF (CSF3) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse G-CSF (CSF3) with 5.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Example of G-CSF standard curve for Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Example of G-CSF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native G-CSF in mouse serum and plasma (citrate) samples.
The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Interpolated concentrations of native G-CSF in RAW264.7 LPSstimulated cell culture supernatant samples.
RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
LPS-treatment of RAW 264.7 cells stimulates secretion of G-CSF.
RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. Various dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of G-CSF adjusted for sample dilution are graphed in pg of G-CSF per mL of supernatant (mean +/- SD, n = 2). Note that G-CSF is not detectable in untreated RAW 264.7 supernatant samples.
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