AB119595

Mouse Galectin 1 ELISA Kit

Name: Mouse Galectin 1 ELISA Kit (ab119595) | Abcam
Brand: Abcam Limited
SKU: AB119595
Price: 780 USD
Availability: InStock

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(3 Publications)

Mouse Galectin 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Galectin 1 in Mouse in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

Gbp, Lgals1, Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1

1 Images

Sandwich ELISA - Mouse Galectin 1 ELISA Kit (AB119595)

sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin 1 ELISA Kit (AB119595)

Representative Standard Curve using ab119595

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's mouse Galectin 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Galectin 1 in cell culture supernatants, serum and plasma (heparin, EDTA).

A Galectin 1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Galectin 1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Galectin 1 amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "318 pg/mL", "standardDeviation": "20.67", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "343 pg/mL", "standardDeviation": "22.29", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1697 pg/mL", "standardDeviation": "144.24", "coefficientOfVariability": "= 8.5" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4684 pg/mL", "standardDeviation": "304.46", "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1747 pg/mL", "standardDeviation": "132.77", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4774 pg/mL", "standardDeviation": "300.76", "coefficientOfVariability": "= 8.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119595-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119595-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse Galectin-1 antibody", "number":"AB119595-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse Galectin 1 antibody Microplate (12 x 8 wells)", "number":"AB119595-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119595-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119595-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119595-CMP06", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119595-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119595-CMP07", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse Galectin 1 standard", "number":"AB119595-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119595-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119595-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.

Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.

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Product protocols

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Target data

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.

See full target information Lgals1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 9:252 PubMed31355153

2019

Thymic Microenvironment Is Modified by Malnutrition and Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Monica Losada-Barragán,Adriana Umaña-Pérez,Jonathan Durães,Sergio Cuervo-Escobar,Andrés Rodríguez-Vega,Flávia L Ribeiro-Gomes,Luiz R Berbert,Fernanda Morgado,Renato Porrozzi,Daniella Arêas Mendes-da-Cruz,Priscila Aquino,Paulo C Carvalho,Wilson Savino,Myriam Sánchez-Gómez,Gabriel Padrón,Patricia Cuervo

Frontiers in physiology 9:689 PubMed29922180

2018

Increased Reactive Oxygen Species and Cell Cycle Defects Contribute to Anemia in the RASA3 Mutant Mouse Model s.

Applications

Unspecified application

Species

Unspecified reactive species

Emily S Hartman,Elena C Brindley,Julien Papoin,Steven L Ciciotte,Yue Zhao,Luanne L Peters,Lionel Blanc

Scientific reports 7:16168 PubMed29170525

2017

Advanced glycation endproducts link inflammatory cues to upregulation of galectin-1 in diabetic retinopathy.

Applications

FuncS

Species

Mouse

Atsuhiro Kanda,Yoko Dong,Kousuke Noda,Wataru Saito,Susumu Ishida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Galectin 1 ELISA Kit

Sandwich ELISA - Mouse Galectin 1 ELISA Kit (AB119595)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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