AB253212

Mouse Galectin-1 ELISA Kit

Recombinant
SimpleStep
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Mouse Galectin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Galectin-1 with a sensitivity of 0.17 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Gbp, Lgals1, Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1

2 Images

Sandwich ELISA - Mouse Galectin-1 ELISA Kit (AB253212)

sELISA

Unknown

Sandwich ELISA - Mouse Galectin-1 ELISA Kit (AB253212)

Interpolated concentrations of native Galectin-1 in mouse serum and plasma samples.

The concentrations of Galectin-1 were measured in duplicates, interpolated from the Galectin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 31.5 ng/mL in serum, 30.4 ng/mL in plasma (citrate) and 28.3 ng/mL in plasma (heparin).

sELISA

Unknown

Sandwich ELISA - Mouse Galectin-1 ELISA Kit (AB253212)

Example of mouse Galectin-1 standard curve in Sample Diluent NS.

The Galectin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum

Reacts with

Mouse, Cow

Assay type

Sandwich (quantitative)

Sensitivity

= 0.17 ng/mL

Range

0.19 - 12 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Galectin-1 ELISA Kit (ab253212) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-1 protein in hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Galectin-1 with 0.17 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Galectin-1, also known as 14 kDa lectin, galaptin, and lectin galactoside-binding soluble 1, is a β-galactoside binding lectin encoded by the gene LGALS1. Galectin-1 is a 134 amino acid non-glycosylated secreted protein that forms a non-covalently linked homodimer. Galectin-1 is expressed in a wide range of tissues and has various intra- and extra-cellular functions, where generally it is a modulator of immune responses. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Serum", "range": "105 - 113 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "114 - 129 %", "average": "= 123" }, { "sample": "Citrate plasma", "range": "104 - 123 %", "average": "= 113" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.

Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.

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Product protocols

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Target data

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.

See full target information Lgals1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Galectin-1 ELISA Kit

Sandwich ELISA - Mouse Galectin-1 ELISA Kit (AB253212)

Sandwich ELISA - Mouse Galectin-1 ELISA Kit (AB253212)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise