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AB206988

Mouse Galectin-7 ELISA Kit

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Mouse Galectin-7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Galectin-7 with a sensitivity of 2.6 pg/well. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Galectin-7, Gal-7, Lgals7

4 Images
Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)

Example of mouse Galectin-7 standard curve in 1X Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)

Example of mouse Galectin-7 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)

Interpolated concentrations of Galectin-7 in mouse serum, mouse plasma (EDTA), and mouse plasma (heparin).

The concentrations of galectin-7 were measured in duplicate, interpolated from the Galectin-7 standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-7 concentration was determined to be 5.07 ng/mL in neat mouse serum, 3.56 ng/mL in neat mouse plasma (EDTA), and 4.36 ng/mL in neat mouse plasma (Heparin).

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Galectin-7 ELISA Kit (AB206988)

Interpolated concentrations of Galectin-7 in mouse skin and esophagus extracts.

The concentrations of Galectin-7 were measured in duplicate, interpolated from the Galectin-7 standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean galectin-7 concentration was determined to be 2.06 ng/mL in 2 μg/mL mouse skin extract and 1.39 ng/mL in 5 μg/mL mouse esophagus extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.6 pg/well

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Galectin-7 ELISA Kit (ab206988) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-7 protein in hep plasma, serum, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Galectin-7 with 2.6 pg/well sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Galectin-7 is a 15kDa monomeric member of the galectin family of carbohydrate-binding proteins. Galectin-7 is primarily expressed in epithelial cells. It is hypothesized to have roles in cell-cell and/or cell-matrix interactions necessary for normal growth control.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 109 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "102 - 105 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "101 - 113 %", "average": "= 107" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab206988-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB206988-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB206988-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB206988-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB206988-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB206988-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Galectin-7 Detector Antibody", "number":"AB206988-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB206988-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB206988-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB206988-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB206988-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Galectin-7 Capture Antibody", "number":"AB206988-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Galectin-7 Lyophilized Recombinant Protein", "number":"AB206988-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 7 also known as Gal-7 is a member of the galectin family of beta-galactoside-binding proteins. It has a molecular mass of approximately 15 kDa. Structurally Galectin 7 has a single carbohydrate recognition domain (CRD) which allows it to bind glycoconjugates. Galectin 7 expresses in various tissues with high expression levels in the epidermis and in stratified squamous epithelia.
Biological function summary

Galectin 7 plays a role in cell-cell adhesion apoptosis regulation and immune response modulation. It plays a role in the cell's apoptosis machinery and interacts with the cytochrome c release pathway. This protein does not typically form a part of large complexes functioning individually in most biological processes. It can influence keratinocyte migration indicating involvement in wound healing processes.

Pathways

Galectin 7 participates in the apoptosis and cell adhesion pathways. It works alongside proteins such as Bcl-2 and Bax to regulate programmed cell death by promoting apoptosis. Additionally it interacts with matrix metalloproteinases influencing the remodelling of extracellular matrix a critical component of tissue repair and immune response.

Galectin 7 is associated with cancer and psoriasis. In cancer it can act as a pro-apoptotic factor and modulates tumor progression often in concert with p53 a well-known tumor suppressor protein. In psoriasis aberrant expression of Galectin 7 correlates with impaired epidermal function and inflammation suggesting it may alter the disease course by influencing local immune responses and keratinocyte behavior.
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Product protocols

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Target data

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release (By similarity).
See full target information Lgals7
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websiteProtocolBooklet
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Product promise

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