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AB155447

Mouse GAS6 ELISA Kit

5

(1 Review)

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(1 Publication)

Mouse GAS6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse GAS6 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Growth arrest-specific protein 6, GAS-6, AXL receptor tyrosine kinase ligand, Gas6

2 Images
Sandwich ELISA - Mouse GAS6 ELISA Kit (AB155447)
  • sELISA

Unknown

Sandwich ELISA - Mouse GAS6 ELISA Kit (AB155447)

Representative standard curve using ab155447 - Assay Diluent B.

Sandwich ELISA - Mouse GAS6 ELISA Kit (AB155447)
  • sELISA

Unknown

Sandwich ELISA - Mouse GAS6 ELISA Kit (AB155447)

Representative standard curve using ab155447 - Assay Diluent A.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

20.48 - 5000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's GAS6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse GAS6 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse GAS6 coated on a 96-well plate. Standards and samples are pipetted into the wells and GAS6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GAS6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GAS6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "68 - 81 %", "average": "= 75.6" }, { "sample": "Serum", "range": "110 - 142 %", "average": "= 124.6" }, { "sample": "Plasma", "range": "109 - 130 %", "average": "= 120.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155447-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155447-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155447-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155447-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB155447-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse GAS6", "number":"AB155447-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155447-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse GAS6 Standard (lyophilized)", "number":"AB155447-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155447-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"GAS6 Microplate (12 x 8 wells)", "number":"AB155447-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155447-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GAS6 also known as Growth arrest-specific 6 is a secreted protein with a molecular mass of approximately 75 kDa. It belongs to the Gla domain-containing protein family. GAS6 expresses in several tissues including the lung heart and kidney with a higher expression in cells undergoing growth arrest. The protein functions mainly through binding to the tyrosine kinase receptors Axl Sky and Mer participating in a wide range of cellular processes.
Biological function summary

GAS6 plays an important role in cell survival proliferation and apoptosis. It is part of the TAM receptor complex along with proteins Axl Mer and Tyro3. This complex mediates several physiological functions including immune response modulation and phagocytosis of apoptotic cells. Besides GAS6 influences cell migration and adhesion making it a significant player in tissue homeostasis and repair.

Pathways

The interactions involving GAS6 include the PI3K/AKT signaling pathway and JAK/STAT pathway. In the PI3K/AKT pathway GAS6 promotes cell survival and growth by activating downstream targets in response to extracellular signals. Through the JAK/STAT pathway GAS6 contributes to cytokine signaling and immune function. Its association with the Axl receptor is particularly notable directly influencing these pathways and expanding on their regulatory mechanisms.

GAS6 has been linked to cancer and inflammatory diseases. Its overexpression often occurs in certain types of cancer such as breast and pancreatic cancers where it can promote tumor progression through the Axl signaling pathway. In inflammatory diseases GAS6 interacts with the Mer receptor contributing to the resolution of inflammation and prevention of autoimmune disorders. Therapeutic research focuses on targeting GAS6 and its receptors as potential treatments for these diseases.
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Product protocols

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Target data

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
See full target information Gas6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed30998506

2019

Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Frisch,Corey M Hoffman,Sarah E Latchney,Mark W LaMere,Jason Myers,John Ashton,Allison J Li,Jerry Saunders,James Palis,Archibald S Perkins,Amanda McCabe,Julianne Np Smith,Kathleen E McGrath,Fatima Rivera-Escalera,Andrew McDavid,Jane L Liesveld,Vyacheslav A Korshunov,Michael R Elliott,Katherine C MacNamara,Michael W Becker,Laura M Calvi
View all publications
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websiteProtocolBooklet
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