AB246544

Mouse GC-C ELISA Kit

Recombinant
SimpleStep
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Mouse GC-C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GC-C with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Guc2c, Gucy2c, Guanylyl cyclase C, GC-C, Heat-stable enterotoxin receptor, Intestinal guanylate cyclase, STA receptor

2 Images

Sandwich ELISA - Mouse GC-C ELISA Kit (AB246544)

sELISA

Supplier Data

Sandwich ELISA - Mouse GC-C ELISA Kit (AB246544)

Interpolated concentrations of native GC-C in mouse small intestine and colon extract.

The concentrations of GC-C were measured in duplicate and interpolated from the GC-C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse GC-C ELISA Kit (AB246544)

Example of mouse GC-C standard curve in 1X Cell Extraction buffer PTR.

The GC-C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse GC-C ELISA Kit (ab246544) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GC-C protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GC-C with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "84 - 99 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246544-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246544-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB246544-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246544-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246544-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB246544-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GC-C Detector Antibody", "number":"AB246544-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246544-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246544-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246544-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GC-C Capture Antibody", "number":"AB246544-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse GC-C Lyophilized Recombinant Protein", "number":"AB246544-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GC-C also known as guanylate cyclase C is an enzyme with mass around 120 kDa expressed mainly in the intestinal epithelium. This membrane-bound receptor plays an important role in the regulation of fluid and electrolyte balance in the intestine. GC-C gets activated by its ligands including bacterial heat-stable enterotoxins as well as endogenous peptides like guanylin and uroguanylin. Upon activation GC-C catalyzes the conversion of GTP to cGMP thereby initiating a signaling cascade that affects ion transport in intestinal cells.

Biological function summary

GC-C is part of a signaling mechanism involved in maintaining fluid balance and gut homeostasis. The enzyme participates in the cGMP-mediated signaling pathway essential for modulating chloride and bicarbonate ion secretion leading to water loss into the intestinal lumen. GC-C does not work in isolation but cooperates with other molecules to facilitate these functions. Specifically it associates with ion transporters and channels influencing the enteric nervous system.

Pathways

GC-C functions within the cGMP pathway which is critical for intestinal fluid regulation. GC-C also connects with the Wnt signaling pathway an important metabolic relay influencing cell proliferation and differentiation. By producing cGMP GC-C influences down-stream proteins such as protein kinase G (PKG) and cystic fibrosis transmembrane conductance regulator (CFTR). These proteins play pivotal roles in maintaining the physiological processes in the intestine.

GC-C relates to irritable bowel syndrome (IBS) and colorectal cancer. Overactivation of GC-C by bacterial toxins can lead to enterotoxigenic diarrhea common in IBS. Its role in colorectal cancer arises from dysregulated signaling pathways affecting cellular growth. Mutations and alterations in GC-C expression may contribute to cancer progression. Furthermore GC-C-related dysregulation can involve other proteins like E-cadherin which modulates cell adhesion in tumor pathways complicating disease dynamics.

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Product protocols

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Target data

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP.

See full target information Gucy2c

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse GC-C ELISA Kit

Sandwich ELISA - Mouse GC-C ELISA Kit (AB246544)

Sandwich ELISA - Mouse GC-C ELISA Kit (AB246544)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise