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AB171178

Mouse GDNF ELISA Kit

5

(1 Review)

|

(8 Publications)

Mouse GDNF ELISA Kit is a Sandwich ELISA for the measurement of Mouse GDNF in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Glial cell line-derived neurotrophic factor, mGDNF, Astrocyte-derived trophic factor, ATF, Gdnf

1 Images
Functional Studies - Mouse GDNF ELISA Kit (AB171178)
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Functional Studies - Mouse GDNF ELISA Kit (AB171178)

Representative standard curve using ab171178.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Microplate

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Primary Antibodies

AB176564

Anti-GDNF antibody [EPR2714(N)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GDNF antibody [EPR2714(N)] (AB176564)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's mouse GDNF in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse GDNF in cell culture supernatants, serum and plasma (heparin, EDTA).

A GDNF specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for GDNF is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse GDNF amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171178-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171178-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171178-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-mouse GDNF antibody", "number":"AB171178-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse GDNF antibody Microplate (12 x 8 wells)", "number":"AB171178-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171178-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171178-CMP10", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171178-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171178-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171178-CMP08", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant mouse GDNF standard", "number":"AB171178-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171178-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. Involved in the development of the neural crest.
See full target information Gdnf
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 19:e3001350 PubMed34748545

2021

Adult medial habenula neurons require GDNF receptor GFRα1 for synaptic stability and function.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Fernández-Suárez,Favio A Krapacher,Katarzyna Pietrajtis,Annika Andersson,Lilian Kisiswa,Alvaro Carrier-Ruiz,Marco A Diana,Carlos F Ibáñez

International journal of molecular sciences 22: PubMed34445251

2021

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Shyuan Rau,Pao-Jen Kuo,Shao-Chun Wu,Lien-Hung Huang,Tsu-Hsiang Lu,Yi-Chan Wu,Chia-Jung Wu,Chia-Wei Lin,Chia-Wen Tsai,Ching-Hua Hsieh

Cell transplantation 29:963689720960185 PubMed33028107

2020

Transplantation of Adipose-Derived Stem Cells Alleviates Striatal Degeneration in a Transgenic Mouse Model for Multiple System Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Chang,Jen-Wei Liu,Bo Cheng Chen,Zhe Sheng Jiang,Chi Tang Tu,Che Hung Su,Hsin Han Yang,Zong Qi Liu,Yu Chen Deng,Chih Yu Chen,Sheng-Tzung Tsai,Shinn Zong Lin,Tzyy-Wen Chiou

Molecular therapy : the journal of the American Society of Gene Therapy 28:642-652 PubMed31495777

2019

SINEUP Non-coding RNA Targeting GDNF Rescues Motor Deficits and Neurodegeneration in a Mouse Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Espinoza,Margherita Scarpato,Devid Damiani,Francesca Managò,Maddalena Mereu,Andrea Contestabile,Omar Peruzzo,Piero Carninci,Claudio Santoro,Francesco Papaleo,Federico Mingozzi,Giuseppe Ronzitti,Silvia Zucchelli,Stefano Gustincich

Glia 66:445-458 PubMed29105838

2017

A Parkinson's disease gene, DJ-1, repairs brain injury through Sox9 stabilization and astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Joo Choi,Jin-Hwa Eun,Byung Gon Kim,Ilo Jou,Sang Myun Park,Eun-Hye Joe

Scientific reports 6:36779 PubMed27827452

2016

GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Wei,Yuanyuan Jia,Yuanyuan Xue,Lei Geng,Min Wang,Lufan Li,Mei Wang,Xuemei Zhang,Xin Wu

Molecules (Basel, Switzerland) 21: PubMed27490526

2016

Agavins Increase Neurotrophic Factors and Decrease Oxidative Stress in the Brains of High-Fat Diet-Induced Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Franco-Robles,Mercedes G López

PloS one 9:e100196 PubMed24949623

2014

Morphine enhances HIV-1SF162-mediated neuron death and delays recovery of injured neurites.

Applications

Unspecified application

Species

Unspecified reactive species

Ruturaj R Masvekar,Nazira El-Hage,Kurt F Hauser,Pamela E Knapp
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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