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AB233621

Mouse GFAP ELISA Kit

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(7 Publications)

Mouse GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GFAP with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Glial fibrillary acidic protein, GFAP, Gfap

4 Images
ELISA - Mouse GFAP ELISA Kit (AB233621)
  • ELISA

Supplier Data

ELISA - Mouse GFAP ELISA Kit (AB233621)
Sandwich ELISA - Mouse GFAP ELISA Kit (AB233621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse GFAP ELISA Kit (AB233621)

Interpolated concentrations of native GFAP in mouse brain extract based on a 2.5 μg/mL extract load.

The concentrations of GFAP were measured in duplicate and interpolated from the GFAP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GFAP concentration was determined to be 2931.53 pg/mL in mouse brain extract.

Sandwich ELISA - Mouse GFAP ELISA Kit (AB233621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse GFAP ELISA Kit (AB233621)

Tissue specificity was assessed by comparison of raw signal in mouse brain, heart, and liver extracts.

Low (2.5 μg/ml) and high (50 μg/ml) concentrations of mouse heart and liver extracts were compared to the brain tissue Heart and liver extracts do not produce signal above background.

Sandwich ELISA - Mouse GFAP ELISA Kit (AB233621)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse GFAP ELISA Kit (AB233621)

Example of mouse GFAP standard curve in 1x Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 8.7 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse GFAP ELISA Kit ab233621 is a rapid single-wash 90-min ELISA kit to measure Mouse GFAP in tissue extracts. This SimpleStep ELISA® sensitivity is 8.7 pg/mL.

How the assay works
Mouse GFAP SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant mouse GFAP protein in tissue extract samples only.

Mouse GFAP ELISA Kit ab233621 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse GFAP ELISA Kit ab233621
Mouse GFAP ELISA Kit ab233621 has been used to develop a nanosensor to identify mild traumatic brain injury (1), to test resolvin D2 as a therapy for neuropathic pain and bone cancer pain (2), and to study clearance of microglial debris for CNS homeostasis (3).

References:
(1)Ester J Kwon et al. 2023, PMID: 37873031,
(2)Xiaopeng Wang et al. 2023, PMID: 36672133,
(3)Bo Peng, et al. 2022, PMID: 36280666

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Mouse GFAP Antibody Pair - BSA and Azide free ab244094
- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) ab242692
- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector) ab242937

Related and recommended products
Mouse GFAP ELISA Kit ab233621 is commonly used to measure neuroinflammatory diseases such as Alzheimer's Disease and traumatic brain injury.

Other ELISA kits to measure neuroinflammation include:
- Mouse IL-6 ELISA kit ab222503
- Mouse TNF alpha ELISA kit ab208348
- Mouse IL-1 beta ELISA Kit ab197742

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Tissue Extracts", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Tissue Extracts", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "95 - 100 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233621-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB233621-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233621-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233621-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GFAP Capture Antibody", "number":"AB233621-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse GFAP Lyophilized Recombinant Protein", "number":"AB233621-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB233621-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233621-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233621-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233621-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GFAP Detector Antibody", "number":"AB233621-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233621-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information Gfap
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:156 PubMed38872143

2024

Profiling the neuroimmune cascade in 3xTg-AD mice exposed to successive mild traumatic brain injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa F Pybus,Sara Bitarafan,Rowan O Brothers,Alivia Rohrer,Arushi Khaitan,Felix Rivera Moctezuma,Kareena Udeshi,Brae Davies,Sydney Triplett,Martin N Griffin,Eric B Dammer,Srikant Rangaraju,Erin M Buckley,Levi B Wood

Advanced functional materials 33: PubMed37873031

2023

An Activity-Based Nanosensor for Minimally-Invasive Measurement of Protease Activity in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Kudryashev,Marianne I Madias,Rebecca M Kandell,Queenie X Lin,Ester J Kwon

Brain sciences 13: PubMed36672133

2023

Resolvin D2 Reduces Chronic Neuropathic Pain and Bone Cancer Pain via Spinal Inhibition of IL-17 Secretion, CXCL1 Release and Astrocyte Activation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Pang,Pengfei Xin,Ying Kong,Zhe Wang,Xiaopeng Wang

Nature communications 13:6233 PubMed36280666

2022

Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Zhou,Yuxin Li,Xiaoyu Li,Fanzhuo Zeng,Yanxia Rao,Yang He,Yafei Wang,Meizhen Liu,Dali Li,Zhen Xu,Xin Zhou,Siling Du,Fugui Niu,Jiyun Peng,Xifan Mei,Sheng-Jian Ji,Yousheng Shu,Wei Lu,Feifan Guo,Tianzhun Wu,Ti-Fei Yuan,Ying Mao,Bo Peng

Alzheimer's research & therapy 13:58 PubMed33678186

2021

Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Emily B Button,Wai Hang Cheng,Carlos Barron,Honor Cheung,Asma Bashir,Jennifer Cooper,Jasmine Gill,Sophie Stukas,David C Baron,Jerome Robert,Elyn M Rowe,Peter A Cripton,Cheryl L Wellington

Folia neuropathologica 58:176-193 PubMed32729296

2020

Tanshinone IIA alleviates the damage of neurocytes by targeting GLUT1 in ischaemia reperfusion model (in vivo and in vitro experiments).

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Haibo Tong,Xiangyang Wang,Xinxing Wang,Yang Wang

Theranostics 10:1604-1618 PubMed32042325

2020

Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Dallan McMahon,Wendy Oakden,Kullervo Hynynen
View all publications
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