Mouse GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GFAP with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Images
Publications
Filter by
- Advanced functional materials 33:2023An Activity-Based Nanosensor for Minimally-Invasive Measurement of Protease Activity in Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia A Kudryashev,Marianne I Madias,Rebecca M Kandell,Queenie X Lin,Ester J KwonPubMed 37873031
- Brain sciences 13:2023Resolvin D2 Reduces Chronic Neuropathic Pain and Bone Cancer Pain via Spinal Inhibition of IL-17 Secretion, CXCL1 Release and Astrocyte Activation in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Pang,Pengfei Xin,Ying Kong,Zhe Wang,Xiaopeng WangPubMed 36672133
- Nature communications 13:62332022Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zhou,Yuxin Li,Xiaoyu Li,Fanzhuo Zeng,Yanxia Rao,Yang He,Yafei Wang,Meizhen Liu,Dali Li,Zhen Xu,Xin Zhou,Siling Du,Fugui Niu,Jiyun Peng,Xifan Mei,Sheng-Jian Ji,Yousheng Shu,Wei Lu,Feifan Guo,Tianzhun Wu,Ti-Fei Yuan,Ying Mao,Bo PengPubMed 36280666
- Alzheimer's research & therapy 13:582021Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily B Button,Wai Hang Cheng,Carlos Barron,Honor Cheung,Asma Bashir,Jennifer Cooper,Jasmine Gill,Sophie Stukas,David C Baron,Jerome Robert,Elyn M Rowe,Peter A Cripton,Cheryl L WellingtonPubMed 33678186
- Folia neuropathologica 58:176-1932020Tanshinone IIA alleviates the damage of neurocytes by targeting GLUT1 in ischaemia reperfusion model (in vivo and in vitro experiments).Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Haibo Tong,Xiangyang Wang,Xinxing Wang,Yang WangPubMed 32729296
- Theranostics 10:1604-16182020Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDallan McMahon et. al.PubMed 32042325
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 8.7 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP, Gfap
What's included?
Recommended products
Mouse GFAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GFAP with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 8.7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Tissue Extracts | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Tissue Extracts | n 3 | mean - | SD - | C.V. 5.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 97 | Range 95 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse GFAP ELISA Kit ab233621 is a rapid single-wash 90-min ELISA kit to measure Mouse GFAP in tissue extracts. This SimpleStep ELISA® sensitivity is 8.7 pg/mL.
How the assay works
Mouse GFAP SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant mouse GFAP protein in tissue extract samples only.
Mouse GFAP ELISA Kit ab233621 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Mouse GFAP ELISA Kit ab233621
Mouse GFAP ELISA Kit ab233621 has been used to develop a nanosensor to identify mild traumatic brain injury (1), to test resolvin D2 as a therapy for neuropathic pain and bone cancer pain (2), and to study clearance of microglial debris for CNS homeostasis (3).
References:
(1)Ester J Kwon et al. 2023, PMID: 37873031,(2)Xiaopeng Wang et al. 2023, PMID: 36672133,
(3)Bo Peng, et al. 2022, PMID: 36280666
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Mouse GFAP Antibody Pair - BSA and Azide free Human/Mouse GFAP Antibody Pair - BSA and Azide free ab244094
- Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) ab242692
- Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector) Anti-GFAP antibody [EPR19996] - BSA and Azide free (Detector) ab242937
Related and recommended products
Mouse GFAP ELISA Kit ab233621 is commonly used to measure neuroinflammatory diseases such as Alzheimer's Disease and traumatic brain injury.
Other ELISA kits to measure neuroinflammation include:
- Mouse IL-6 ELISA kit Mouse IL-6 ELISA Kit ab222503
- Mouse TNF alpha ELISA kit Mouse TNF alpha ELISA Kit ab208348
- Mouse IL-1 beta ELISA Kit Mouse IL-1 beta ELISA Kit ab197742
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Mouse GFAP ELISA Kit (ab233621)
Example of mouse GFAP standard curve in 1x Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse GFAP ELISA Kit (ab233621)
Interpolated concentrations of native GFAP in mouse brain extract based on a 2.5 μg/mL extract load.
The concentrations of GFAP were measured in duplicate and interpolated from the GFAP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GFAP concentration was determined to be 2931.53 pg/mL in mouse brain extract.
Sandwich ELISA - Mouse GFAP ELISA Kit (ab233621)
Tissue specificity was assessed by comparison of raw signal in mouse brain, heart, and liver extracts.
Low (2.5 μg/ml) and high (50 μg/ml) concentrations of mouse heart and liver extracts were compared to the brain tissue Heart and liver extracts do not produce signal above background.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com