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AB46078

Mouse GM-CSF ELISA Kit

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(2 Publications)

Mouse GM-CSF ELISA Kit is a Sandwich ELISA for the measurement of Mouse GM-CSF in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Csfgm, Csf2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF

1 Images
Sandwich ELISA - Mouse GM-CSF ELISA Kit (AB46078)
  • sELISA

Unknown

Sandwich ELISA - Mouse GM-CSF ELISA Kit (AB46078)

Representative Standard Curve using ab46078

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 9 pg/mL

Range

15.6 - 500 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's GM-CSF mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for GM-CSF has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF present in the samples.

This kit will recognize both endogenous and recombinant mouse GM-CSF.

Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 7, "mean": "271.9 pg/mL", "standardDeviation": "10.8", "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 7, "mean": "65.4 pg/mL", "standardDeviation": "4.3", "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "279.2 pg/mL", "standardDeviation": "14.7", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "62.5 pg/mL", "standardDeviation": "2.52", "coefficientOfVariability": "4" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46078-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46078-CMP03" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46078-CMP02" }, { "size":"4 x 1 Vial", "name":"GM-CSF Standard (Lyophilized)", "number":"AB46078-CMP06" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46078-CMP07" }, { "size":"2 x 1 Unit", "name":"GM-CSF Microplate (12 x 8 well strips)", "number":"AB46078-CMP09" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46078-CMP01" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-GM-CSF", "number":"AB46078-CMP10" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46078-CMP04" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46078-CMP08" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46078-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46078-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46078-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46078-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46078-CMP08", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46078-CMP04", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-GM-CSF", "number":"AB46078-CMP10", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46078-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"GM-CSF Microplate (12 x 8 well strips)", "number":"AB46078-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"GM-CSF Standard (Lyophilized)", "number":"AB46078-CMP06", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46078-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46078-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
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Product protocols

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Target data

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
See full target information Csf2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 16:8434-8440 PubMed28983597

2017

Mediating macrophage immunity with wogonin in mice with vascular inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Wang,Kunxia Li,Yupeng Li,Yulin Wang

The Journal of pathology 230:310-21 PubMed23520016

2013

Distinct roles of CSF family cytokines in macrophage infiltration and activation in glioma progression and injury response.

Applications

Unspecified application

Species

Unspecified reactive species

Malgorzata Sielska,Piotr Przanowski,Bartosz Wylot,Konrad Gabrusiewicz,Marta Maleszewska,Magdalena Kijewska,Malgorzata Zawadzka,Joanna Kucharska,Katyayni Vinnakota,Helmut Kettenmann,Katarzyna Kotulska,Wieslawa Grajkowska,Bozena Kaminska
View all publications
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Product promise

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