Mouse GM-CSF ELISA Kit (CSF2)
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(2 Publications)
Mouse GM-CSF ELISA Kit (CSF2) is a Sandwich ELISA for the measurement of Mouse GM-CSF (CSF2) in Mouse in Biofluids, Cell Culture Media samples.
View Alternative Names
Csfgm, Csf2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF
- sELISA
Supplier Data
Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB100685)
Representative Standard Curve using ab100685
- sELISA
Supplier Data
Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB100685)
Representative Standard Curve using ab100685
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Reactivity data
Product details
Abcam's GM-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse GM-CSF in plasma and cell culture supernatants.
This assay employs an antibody specific for mouse GM-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse GM-CSF in normal serum with ab100685, only in serum spiked with Mouse GM-CSF.
Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.
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Properties and storage information
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Supplementary information
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Biological function summary
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
Pathways
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
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Publications (2)
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Journal for immunotherapy of cancer 11: PubMed36958764
2023
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The Journal of biological chemistry 298:101406 PubMed34774800
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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