Mouse GM-CSF ELISA Kit (CSF2)
- Recombinant
- SimpleStep
- What is this?
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(1 Publication)
Mouse GM-CSF ELISA Kit (CSF2) is a single-wash 90-min Simplestep used to quantify Mouse GM-CSF (CSF2) with a sensitivity of 3.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Csfgm, Csf2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF
- ELISA
Supplier Data
ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB201276)
- sELISA
Lab
Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB201276)
Mouse GM-CSF (CSF2) standard curve comparison data.
Standard curve comparison between mouse GM-CSF (CSF2) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB201276)
Comparison of secreted GM-CSF in unstimulated and LPS stimulated RAW264.7 Cells.
RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 μg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. GM-CSF was measured in undiluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7. Measured values were interpolated from the GM-CSF Standard Curve diluted in Sample Diluent NS. Mean of duplicate values +/-SD are graphed : 4.1 pg/mL unstimulated, 269.5 pg/mL stimulated.
- sELISA
Supplier Data
Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB201276)
Linearity of dilution of the assay. Interpolated concentrations of GM-CSF in mouse cell culture media.
The concentrations of GM-CSF were measured in duplicate and interpolated from the GM-CSF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 289.7 pg/mL in mouse RAW LPS-stimulated supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Mouse GM-CSF ELISA Kit (CSF2) (AB201276)
Example of GM-CSF standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse GM-CSF ELISA Kit (CSF2) ab201276 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse GM-CSF (CSF2)in cell culture supernatant. This SimpleStep sensitivity is 3.1 pg/mL.
How the assay works
Mouse GM-CSF (CSF2)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse GM-CSF ELISA Kit (CSF2) ab201276 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Mouse GM-CSF (CSF-2) is a ~25 kDa pleiotropic cytokine produced by a variety of cells (activated T and B cells, macrophages, mast cells, fibroblasts, endothelial cells) during inflammation or in response to cytokines. GM-CSF can induce proliferation and differentiation of myeloid progenitor cells into eosinophils, macrophages/monocytes, and neutrophils. It is also a survival factor for mature eosinophils, macrophages/monocytes, and neutrophils.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
Pathways
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Advanced therapeutics 4: PubMed34632048
2021
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
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