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AB193724

Mouse gp130 ELISA Kit

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Mouse gp130 ELISA Kit is a Sandwich ELISA for the measurement of Mouse gp130 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD130 antigen, CDw130, IL-6R-beta, Interleukin-6 receptor subunit beta, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin M receptor alpha subunit, gp130

2 Images
Sandwich ELISA - Mouse gp130 ELISA Kit (AB193724)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse gp130 ELISA Kit (AB193724)

Typical Standard Curve in Assay Diluent B using ab193724 gp130 Mouse ELISA Kit.

Sandwich ELISA - Mouse gp130 ELISA Kit (AB193724)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse gp130 ELISA Kit (AB193724)

Typical Standard Curve in Assay Diluent A using ab193724 gp130 Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 40 pg/mL

Range

40 - 10000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's gp130 MouseELISA Kit (ab193724) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse gp130 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for gp130 coated onto a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures gp130 present in the sample. The wells are washed and a biotinylated anti-Mouse gp130 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of gp130 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 107 %", "average": "= 99.02" }, { "sample": "Plasma", "range": "90 - 103 %", "average": "= 97.9" }, { "sample": "Cell culture media", "range": "113 - 123 %", "average": "= 118.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193724-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193724-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193724-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193724-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193724-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse gp-130 Microplate (12 strips x 8 wells)", "number":"AB193724-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse gp-130 detection antibody", "number":"AB193724-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse gp-130 standards (lyophilized)", "number":"AB193724-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193724-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin Concentrate", "number":"AB193724-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.
Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.
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Product protocols

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Target data

See full target information Il6st
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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