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AB253213

Mouse Granzyme A ELISA Kit

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Mouse Granzyme A ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Granzyme A with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ctla-3, Ctla3, Mtsp-1, Gzma, Granzyme A, Autocrine thymic lymphoma granzyme-like serine protease, CTLA-3, Fragmentin-1, T cell-specific serine protease 1, TSP-1

5 Images
Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)
  • sELISA

Unknown

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)

Interpolated concentrations of native Granzyme A in mouse splenocytes culture supernatant samples.

The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 615 pg/mL in neat splenocyte culture supernatant.

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)
  • sELISA

Unknown

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)

Example of mouse Granzyme A standard curve in 1X Cell Extraction Buffer PTR.

The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)
  • sELISA

Unknown

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)

Interpolated concentrations of native Granzyme A in mouse spleen extract based on a 1,000 μg/mL extract load.

The concentrations of Granzyme A were measured in duplicate and interpolated from the Granzyme A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,424 pg/mL in spleen extract.

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)
  • sELISA

Unknown

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)

Example of mouse Granzyme A standard curve in Sample Diluent NS.

The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)
  • sELISA

Unknown

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)

Example of mouse Granzyme A standard curve in Sample Diluent 50BS.

The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 11 pg/mL

Range

39.1 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Granzyme A ELISA Kit (ab253213) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granzyme A protein in cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Granzyme A with 11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Granzyme A, also known as T cell-specific serine protease 1, is a trypsin-like serine protease that forms a disulfide-linked homodimer and is secreted by cytotoxic T lymphocytes and natural killer cells. While Granzyme A is the most abundant serine protease in killer cell granules, it is considered less cytotoxic than Granzyme B, but can activate the caspase-independent cell death with morphological features of apoptosis. Granzyme A targets several nuclear proteins for degradation including, Pro-IL-1beta, NDUFS3, SET, AP1, Histone H1, HMGB2, PARP-1, Ku70, and lamins, thus limiting DNA repair, oxidative repair activity, and disrupting the election transport of mitochondria. Granzyme A is also considered to have extracellular functions including promoting inflammation and degradation of extracellular matrix.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "16" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 109 %", "average": "= 105" }, { "sample": "Serum", "range": "79 - 81 %", "average": "= 80" }, { "sample": "EDTA Plasma", "range": "105 - 114 %", "average": "= 109" }, { "sample": "Tissue Extracts", "range": "76 - 89 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "96 - 99 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253213-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253213-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253213-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB253213-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253213-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253213-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253213-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Granzyme A Lyophilized Recombinant Protein", "number":"AB253213-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB253213-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB253213-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Granzyme A Capture Antibody", "number":"AB253213-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Granzyme A Detector Antibody", "number":"AB253213-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253213-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB253213-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme A also known as CTLA3 or GZMA is a serine protease with a role in immune cell-mediated cytotoxicity. It has a molecular weight of approximately 27 kDa. This protein is primarily expressed in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Granzyme A gets released into the immunological synapse upon recognition of target cells by these immune cells facilitating the induction of target cell death.
Biological function summary

Functions of Granzyme A extend to inducing apoptosis in target cells alongside its role in promoting inflammation. It operates independent of caspases and directly cleaves substrates in the cell to induce cell death making it different from the related Granzyme B. As part of the granzyme-perforin complex it enters target cells via perforin-created pores mediates reactions leading to substrate degradation and promotes DNA damage.

Pathways

Granzyme A gets involved in the granule exocytosis pathway which constitutes an important mechanism for CTLs and NK cells to kill infected or cancerous cells. The protein is also linked to the inflammatory response pathways where it can interact with other serine proteases. Related proteins like perforin and Granzyme B also engage in these pathways and they collectively work to trigger apoptosis and modulate immune responses.

Impairments or dysregulation of Granzyme A function can contribute to autoimmune diseases and certain viral infections. Autoimmune diseases such as lupus may demonstrate altered levels or activity of Granzyme A associated with tissue damage and inflammation. During viral infections Granzyme A in conjunction with perforin and Granzyme B aids in eliminating infected cells but alterations in this balance could lead to ineffective immune responses or excessive tissue damage.
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Product protocols

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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
See full target information Gzma
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websiteProtocolBooklet
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