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AB238265

Mouse Granzyme B ELISA Kit

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(4 Publications)

Mouse Granzyme B ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Granzyme B with a sensitivity of 24.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ctla-1, Ctla1, Gzmb, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, CCP1

4 Images
ELISA - Mouse Granzyme B ELISA Kit (AB238265)
  • ELISA

Supplier Data

ELISA - Mouse Granzyme B ELISA Kit (AB238265)
Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)

Representative image of Mouse Granzyme B ELISA Kit (ab238265)

Components shown from left to right :

- Sample diluent NS

- 10X Mouse Granzyme B Capture Antibody

- 10X Mouse Granzyme B Detector Antibody

- Mouse Granzyme B Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent CPR2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)

Interpolated concentrations of native Granzyme B in mouse treated and untreated spleen supernatant samples. The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curves and corrected for sample dilution. Undiluted samples are as follows : treated mouse spleen supernatant 1 : 67 and untreated mouse spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme B concentration was determined to be 340.6 ng/mL in treated mouse spleen supernatant and 31.7 ng/ml in untreated mouse spleen supernatant.

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)

Example of mouse Granzyme B standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 24.4 pg/mL

Range

188 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Granzyme B ELISA Kit ab238265 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Granzyme B in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 24.4 pg/mL.

How the assay works

Mouse Granzyme B SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Granzyme B ELISA Kit ab238265 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Granzyme B antibody [EPR20130-11] - BSA and Azide free (Capture) ab245072
- Anti-Granzyme B antibody [EPR20130-273] - BSA and Azide free (Detector) ab245072
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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Spleen", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Mouse Spleen", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 101 %", "average": "= 98" }, { "sample": "Serum", "range": "89 - 103 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "103 - 120 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "85 - 96 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238265-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Mouse Granzyme B Lyophilized recombinant Protein", "number":"AB238265-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Granzyme B Capture Antibody", "number":"AB238265-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238265-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB238265-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238265-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238265-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB238265-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238265-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Granzyme B Detector Antibody", "number":"AB238265-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238265-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238265-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
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Product protocols

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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 35705808). It cleaves after Asp (PubMed : 35705808). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (By similarity). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution (By similarity). Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 35705808). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed : 35705808).
See full target information Gzmb
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:443 PubMed40514658

2025

Overcoming acquired immunotherapy resistance in non-small cell lung cancer using ginsenoside Rb1-loaded, peptide-enhanced exosome delivery systems.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyuan Jin,Tanghesi Wuyun,Yu Zhang,Xiaohong Wang,Ling Zhao

Journal of nanobiotechnology 23:303 PubMed40259377

2025

PTEN as a prognostic factor for radiotherapy plus immunotherapy response in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxing Guo,Ming Zhang,Xiaoli Li,Jiashuo Wang

Journal of nanobiotechnology 22:409 PubMed38992688

2024

Targeting CSF1R in myeloid-derived suppressor cells: insights into its immunomodulatory functions in colorectal cancer and therapeutic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tong,Shifeng Qiao,Zhe Dong,Xiaohui Zhao,Xiaxia Du,Wei Niu

Journal of immunology (Baltimore, Md. : 1950) 212:1381-1391 PubMed38416029

2024

Granzyme F: Exhaustion Marker and Modulator of Chimeric Antigen Receptor T Cell-Mediated Cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary L Z Hay,Dale D Kim,Jennifer M Cimons,Jennifer R Knapp,M Eric Kohler,Mary Quansah,Tiffany M Zúñiga,Faye A Camp,Mayumi Fujita,Xiao-Jing Wang,Brian P O'Connor,Jill E Slansky
View all publications
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