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AB326734

Mouse Granzyme B ELISA Kit, Chemiluminescent

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Mouse Granzyme B ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Granzyme B with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16.46 - 36000 pg/ml with a sensitivity of 6.28 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Ctla-1, Ctla1, Gzmb, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, CCP1

4 Images
Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)
  • sELISA

Lab

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)

Standard curve comparison between the Chemiluminescent Mouse Granzyme B SimpleStep ELISA and colorimetric Mouse Granzyme B SimpleStep ELISA (ab238265) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)
  • sELISA

Lab

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)

Dynamic range comparison between the Chemiluminescent Mouse Granzyme B SimpleStep ELISA, colorimetric Mouse Granzyme B SimpleStep ELISA (ab238265), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)
  • sELISA

Lab

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)

Interpolated concentrations of native Granzyme B in mouse treated and untreated spleen supernatant samples. The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curves and corrected for sample dilution. Undiluted samples are as follows : treated mouse spleen supernatant 1 : 67 and untreated mouse spleen supernatant 25%. Mouse spleen supernatant was treated for 3 days in the presence or absence of 50 ng/mL PMA and 500 ng/mL calcium ionomycin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme B concentration was determined to be 340.6 ng/mL in treated mouse spleen supernatant and 31.7 ng/ml in untreated mouse spleen supernatant. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit, Chemiluminescent (AB326734)

Interpolated concentrations of native Granzyme B in mouse treated and untreated spleen supernatant samples. The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curves and corrected for sample dilution. Undiluted samples are as follows : treated mouse spleen supernatant 1 : 67 and untreated mouse spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme B concentration was determined to be 340.6 ng/mL in treated mouse spleen supernatant and 31.7 ng/ml in untreated mouse spleen supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, Cell culture media, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.28 pg/mL

Range

16.46 - 36000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse Granzyme B ELISA Kit, Chemiluminescent (ab326734) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Granzyme B in Cell culture supernatant, Serum, Cell culture media, Citrate plasma with a sensitivity of 6.28 pg/mL.

How the assay works

Mouse Granzyme B ELISA Kit, Chemiluminescent (ab326734) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Granzyme B (ab326734) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Granzyme B antibody [EPR20130-11] - BSA and Aside free (Capture) ab244799
- Anti-Granzyme B antibody [EPR20130-273] - BSA and Aside free (Detector) ab245072
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Precision

[ { "reproducibilityType": "Intra", "sample": "Mouse Spleen", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.9" }, { "reproducibilityType": "Inter", "sample": "Mouse Spleen", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 101 %", "average": "= 101" }, { "sample": "Serum", "range": "89 - 103 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "103 - 120 %", "average": "= 120" }, { "sample": "Citrate plasma", "range": "85 - 96 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 3555842, PubMed : 35705808). It cleaves after Asp (PubMed : 35705808). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (By similarity). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution (By similarity). Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 35705808). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed : 35705808).
See full target information Gzmb
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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