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AB267578

Mouse Gremlin 1 ELISA Kit

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Mouse Gremlin 1 ELISA Kit is a Sandwich ELISA for the measurement of Mouse Gremlin 1 in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Cktsf1b1, Drm, Grem1, Gremlin-1, Down-regulated in Mos-transformed cells protein

1 Images
Sandwich ELISA - Mouse Gremlin 1 ELISA Kit (AB267578)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Gremlin 1 ELISA Kit (AB267578)

Mouse Gremlin 1 ELISA kit example standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.8 ng/mL

Range

0.819 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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AB22138

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Secondary Antibodies

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

AB280888

Anti-Smad2 (phospho S467) antibody [EPR23681-40]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] (AB280888)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Gremlin 1 ELISA Kit is designed for the quantitative determination of Gremlin 1 in cell culture supernatants, plasma and serum samples.

Δ Note: Mouse Gremlin 1 concentration is low in normal serum and plasma samples and may not be detectable in this assay.

Δ Note: Collect plasma using EDTA as an anticoagulant; Heparin and citrate are not recommended in this assay.

This assay employs an antibody specific for mouse Gremlin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Gremlin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Gremlin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Gremlin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS1, Adiponectin, ANG-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, IFN-gamma R1, IL-17B, IL-17B R, IL-22, MIP-1 beta, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGF beta-1, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "69 - 79 %", "average": "= 74.8" }, { "sample": "Cell culture media", "range": "137 - 149 %", "average": "= 144.9" }, { "sample": "Plasma", "range": "80 - 93 %", "average": "= 86.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267578-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267578-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267578-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse Gremlin 1 coated Microplate (12 x 8 wells)", "number":"AB267578-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267578-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse Gremlin 1 Detection Antibody", "number":"AB267578-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267578-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267578-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Gremlin 1 Standard (Lyophilized)", "number":"AB267578-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267578-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gremlin 1 often referred to as Gremlin or gremlin-1 protein acts as an antagonist to bone morphogenetic proteins (BMPs). Commonly known as Gremlin 1 protein or Gremlin rat in certain studies it exhibits a mass of approximately 28 kDa. Researchers find Gremlin 1 expressed in various tissues including the kidney lung and limb bud highlighting its presence in numerous developmental processes. Its presence in these tissues indicates its importance in organogenesis and tissue differentiation.
Biological function summary

Gremlin 1 inhibits the BMP pathway which plays an important role in cellular growth and differentiation. It does not function alone; instead it becomes part of a complex interaction network that modulates cell proliferation and embryonic organ development. Gremlin 1's binding to BMPs prevents them from interacting with their receptors affecting the signaling cascade necessary for normal bone and cartilage development as well as vascular patterning.

Pathways

The regulatory function of Gremlin 1 is essential in the BMP signaling pathway. The well-known TGF-beta signaling pathway is influenced by Gremlin 1 as it regulates the balance of BMP activity. Gremlin 1's interaction with BMP4 a closely related protein exemplifies its impact on the differentiation and proliferation of mesenchymal cells.

Gremlin 1 expression has links to fibrotic diseases such as pulmonary fibrosis and chronic kidney disease. These conditions often involve excessive tissue scarring and dysfunction where Gremlin 1's modulation of the BMP pathway exacerbates the fibrosis process. Additionally its interaction with proteins such as BMP7 during these disease states highlights the potential for therapeutic targeting to reverse or inhibit fibrotic progression.
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Product protocols

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Target data

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop (PubMed : 12808456, PubMed : 15201225). Down-regulates the BMP4 signaling in a dose-dependent manner (PubMed : 15133038). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (By similarity). Acts as inhibitor of monocyte chemotaxis (By similarity).
See full target information Grem1
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websiteProtocolBooklet
en
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Product promise

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