AB272191

Mouse GRO gamma ELISA Kit (CXCL3)

Recombinant
SimpleStep
What is this?

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(2 Publications)

Mouse GRO gamma ELISA Kit (CXCL3) is a single-wash 90-min Simplestep used to quantify Mouse GRO gamma (CXCL3) with a sensitivity of 1.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Gro, Gro1, Mgsa, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51

4 Images

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

sELISA

Supplier Data

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Interpolated concentrations of native GRO gamma in mouse serum, plasma and cell culture supernatant samples.

The concentrations of GRO gamma were measured in duplicates, interpolated from the GRO gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma EDTA (100%) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GRO gamma concentration was determined to be 145.55 pg/mL in serum, 41.66 pg/mL in plasma (citrate), 31.17 pg/ml in plasma (EDTA) and 50.92 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Interpolated concentrations of native GRO gamma in mouse lung culture supernatant and spleen culture supernatant.

The concentrations of GRO gamma were measured in duplicate and interpolated from the GRO gamma standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GRO gamma concentration was determined to be 125 ng/mL in mouse lung supernatant treated with 5mg/ml LPS for 5 days and 18 ng/mL in mouse lung supernatants, mock treatment and 45.25 ng/ml in mouse spleen supernatant treated with 5mg/ml LPS for 5 days. *GRO gamma levels were assayed two times and found to be below the level of detection in mock treated mouse spleen supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Example of mouse GRO gamma standard curve in Sample Diluent NS.

The GRO gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Mouse GRO gamma standard curve comparison.

Standard Curve comparison between the original Mouse GRO gamma SimpleStep ELISA kit (ab206310) and current Mouse GRO gamma SimpleStep ELISA kit (ab272191).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.3 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse GRO gamma ELISA kit (ab272191) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GRO gamma protein in serum, plasmas and tissue culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GRO gamma with 1.3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Chemokines are a group of small structurally related molecules that play fundamental roles in the development, homeostasis, and function of the immune system. GRO gamma (CXCL3) is an 8 kDa proinflammatory member of the chemokine heparin-binding CXC subfamily, or alpha chemokines, and is a ligand for CXCR2. GRO gamma has chemotactic activity for neutrophils and may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Mouse GRO gamma shares 62%, 90% and 63% sequence homology with human, rat and cow, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 103 %", "average": "= 100" }, { "sample": "Serum", "range": "93 - 108 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "86 - 93 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "88 - 101 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "84 - 90 %", "average": "= 87" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272191-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272191-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GRO gamma (CXCL3) Detector Antibody", "number":"AB272191-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB272191-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272191-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272191-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272191-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272191-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272191-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse GRO gamma (CXCL3) Capture Antibody", "number":"AB272191-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse GRO gamma (CXCL3) Lyophilized Recombinant Protein", "number":"AB272191-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRO gamma also known as CXCL3 is a chemokine with a molecular weight of approximately 7.9 kDa. It is a small protein belonging to the CXC chemokine family. GRO gamma is expressed in numerous tissues including epithelial cells fibroblasts and several leukocytes. This protein plays a significant role in chemotactic signaling drawing neutrophils to sites of inflammation and infection acting as both a signal and effector molecule in immune response. Researchers often use CXCL3 ELISA kits and GRO protein assays to quantify its expression levels in biological samples.

Biological function summary

GRO gamma functions as a chemokine that guides neutrophils by binding to its receptor CXCR2. It participates in inflammatory processes and is produced in response to stimuli such as bacterial products and pro-inflammatory cytokines. GRO gamma together with its close family members forms a network facilitating the immune cell recruitment and activation at infection sites. Also this chemokine is included in larger chemokine complexes influencing not just immune cells but also modulating angiogenesis and cellular matrix restructuring.

Pathways

GRO gamma significantly contributes to the chemokine signaling pathway and the NF-kB signaling pathway. In these pathways it interacts tightly with proteins like CXCR2 which is essential for the signaling cascade that results in immune cell migration. It also engages with the MAPK pathways impacting cell proliferation and apoptosis. These involvements make GRO gamma vital for orchestrating the inflammation response in humans.

GRO gamma is linked to chronic inflammatory conditions and cancer. Elevated levels of GRO gamma have been observed in rheumatoid arthritis where its interaction with CXCR2 exacerbates inflammation and tissue damage. In cancer particularly in colorectal cancer GRO gamma plays a role in tumor progression by promoting angiogenesis and cell proliferation interacting with VEGF and other cytokines. Understanding its functions and relationships opens potential avenues for therapeutic interventions targeting chronic diseases and cancer.

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Product protocols

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Target data

Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation (By similarity). Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.

See full target information Cxcl1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4677 PubMed37542037

2023

Targeting of SLC25A22 boosts the immunotherapeutic response in KRAS-mutant colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Zhou,Yao Peng,Fenfen Ji,Huarong Chen,Wei Kang,Lam-Shing Chan,Hongyan Gou,Yufeng Lin,Pingmei Huang,Danyu Chen,Qinyao Wei,Hao Su,Cong Liang,Xiang Zhang,Jun Yu,Chi Chun Wong

iScience 26:106904 PubMed37275530

2023

CD200R signaling contributes to unfavorable tumor microenvironment through regulating production of chemokines by tumor-associated myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cho-Hao Lin,Fatemeh Talebian,Yang Li,Jianmin Zhu,Jin-Qing Liu,Bolin Zhao,Sujit Basu,Xueliang Pan,Xi Chen,Pearlly Yan,William E Carson,Gang Xin,Haitao Wen,Ruoning Wang,Zihai Li,Qin Ma,Xue-Feng Bai

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse GRO gamma ELISA Kit (CXCL3)

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (AB272191)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise