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AB283967

Mouse GSTA1 ELISA Kit

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(1 Publication)

Mouse GSTA1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse GSTA1 in Mouse in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Gsta, Gstya, Gsta1, Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST class-alpha member 1, Glutathione S-transferase Ya, Glutathione S-transferase Ya1

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Sandwich ELISA - Mouse GSTA1 ELISA Kit (AB283967)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse GSTA1 ELISA Kit (AB283967)

Example of Mouse GSTA1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue, Serum, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.25 - 16 ng/mL

Assay time

5h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse GSTA1 ELISA Kit (ab283967) is designed for detection of GSTA1 in mouse plasma, serum, urine, cell lysate and tissue samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse GSTA1 in approximately 5 hours.

A polyclonal antibody specific for mouse GSTA1 has been pre-coated onto a 96-well microplate with removable strips. GSTA1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for mouse GSTA1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTA1 protein also known as Glutathione S-transferase alpha 1 is an important enzyme in detoxification processes in the body. It has a molecular mass of approximately 25 kDa and expresses highly in the liver but is also seen in other tissues like the kidney and lung. It belongs to the glutathione S-transferase family which plays function in the conjugation of reduced glutathione to a variety of endogenous and exogenous electrophilic compounds.
Biological function summary

The GSTA1 protein contributes significantly to the neutralization of oxidative stress by detoxifying harmful compounds. This enzyme shows a high affinity for certain substrates and serves as a detoxifying agent particularly in the liver safeguarding cells from oxidative damage. GSTA1 exists mostly as a homodimer but can also form heterodimers with other related enzymes enhancing its substrate range and activity.

Pathways

GSTA1 plays a role in metabolic and detoxification pathways essential for cellular protection. It is an important player in the glutathione metabolism pathway where it collaborates with other enzymes like GSTM1 to facilitate detoxification processes. Additionally GSTA1 participates in the pathways leading to the synthesis of mercapturic acids which are important for the excretion of toxic metabolites.

GSTA1 is linked to conditions such as cancer and cardiovascular diseases. Altered expression or polymorphisms in the GSTA1 gene can affect the enzyme's ability to detoxify carcinogens making it significant in cancer risk and progression. The GSTA1 protein also interacts with other enzymes like GSTA4 in the context of oxidative stress which relates to cardiovascular disorder pathogenesis.
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Product protocols

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Target data

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (PubMed : 9606968). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (By similarity).
See full target information Gsta1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 10:e15352 PubMed35748049

2022

Early loss of glutathione -s- transferase (GST) activity during diverse forms of acute renal tubular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Richard A Zager,Ali C M Johnson
View all publications
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websiteProtocolBooklet
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