Mouse H-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse H-FABP with a sensitivity of 1.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.81 - 500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Alternative names
Fabph1, Fabp3, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, Mammary-derived growth inhibitor, H-FABP, MDGI
What's included?
Recommended products
Mouse H-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse H-FABP with a sensitivity of 1.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.81 - 500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Heart Extract | n 8 | mean - | SD - | C.V. 5.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Heart Extract | n 3 | mean - | SD - | C.V. 5.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 100 | Range 94 - 105 % |
Sample type EDTA Plasma | Average % = 99 | Range 97 - 101 % |
Sample type Urine | Average % = 108 | Range 105 - 114 % |
Sample type Tissue Extracts | Average % = 110 | Range 104 - 116 % |
Sample type Heparin Plasma | Average % = 93 | Range 82 - 101 % |
Sample type Citrate plasma | Average % = 101 | Range 100 - 102 % |
Sample type Cell culture media | Average % = 107 | Range 105 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse H-FABP ELISA Kit (ab242240) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of H-FABP protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse H-FABP with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
H-FABP also known as heart-type fatty acid binding protein is a small protein with a molecular mass of approximately 14.5 kDa. This protein is primarily found in the cytoplasm of cardiac muscle cells but it also can be detected in skeletal muscles and other tissues at lower concentrations. H-FABP binds long-chain fatty acids and is important for the intracellular transport and metabolism of these molecules. The protein is often used as a biomarker for cardiac injury due to its rapid release into the bloodstream following muscle damage.
- Biological function summary
Fatty acid binding largely defines H-FABP's role facilitating the transport of fatty acids within cells which is key for energy production. It is not part of a larger protein complex but plays into broader lipid metabolism processes. The efficiency of H-FABP in fatty acid binding and transport helps maintain cellular energy homeostasis especially in tissues with high fatty acid metabolism like the heart.
- Pathways
H-FABP integrates into the fatty acid metabolic pathways and the beta-oxidation pathway. It is associated with the transport and utilization of fatty acids linking it to the peroxisome proliferator-activated receptor (PPAR) signaling pathway an important regulator of lipid metabolism. Proteins such as CD36 and PPARα interact with H-FABP within these pathways influencing lipid storage and energy balance.
- Associated diseases and disorders
Disruptions in H-FABP levels or function correlate with cardiovascular diseases including myocardial infarction. Elevated blood levels of H-FABP provide early markers of cardiac cell injury. Additionally alterations can also relate to metabolic disorders like obesity where lipid metabolism gets impaired. In these contexts proteins such as troponin and creatine kinase may also serve as biomarkers elucidating the relationship between H-FABP and myocardial stress or damage.
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4 product images
Sandwich ELISA - Mouse H-FABP ELISA Kit (ab242240)
Example of mouse H-FABP standard curve in 1X Cell Extraction Buffer PTR.
The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse H-FABP ELISA Kit (ab242240)
Example of mouse H-FABP standard curve in Sample Diluent NS.
The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse H-FABP ELISA Kit (ab242240)
Interpolated concentrations of native H-FABP in mouse serum, plasma and urine samples.
The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:100, plasma (EDTA) 1:100, plasma (heparin) 1:100, and urine 1:8. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 42.1 ng/mL in serum, 69.1 ng/mL in plasma (citrate), 57.2 ng/mL in plasma (EDTA), 76.5 ng/mL in plasma (heparin), and 3.4 ng/mL in urine.
Sandwich ELISA - Mouse H-FABP ELISA Kit (ab242240)
Interpolated concentrations of native H-FABP in mouse heart extract and skeletal muscle extract based on 100 ng/mL and 250 ng/mL extract loads, respectively.
The concentrations of H-FABP were measured in duplicate and interpolated from the H-FABP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP3 concentration was determined to be 427.5 pg/mL in heart extract and 344.3 pg/mL in skeletal muscle extract.
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