Mouse H-FABP ELISA Kit
- Recombinant
- SimpleStep
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Mouse H-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse H-FABP with a sensitivity of 1.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Fabph1, Fabp3, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, Mammary-derived growth inhibitor, H-FABP, MDGI
- sELISA
Supplier Data
Sandwich ELISA - Mouse H-FABP ELISA Kit (AB242240)
Example of mouse H-FABP standard curve in Sample Diluent NS.
The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse H-FABP ELISA Kit (AB242240)
Interpolated concentrations of native H-FABP in mouse serum, plasma and urine samples.
The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 100, plasma (EDTA) 1 : 100, plasma (heparin) 1 : 100, and urine 1 : 8. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 42.1 ng/mL in serum, 69.1 ng/mL in plasma (citrate), 57.2 ng/mL in plasma (EDTA), 76.5 ng/mL in plasma (heparin), and 3.4 ng/mL in urine.
- sELISA
Supplier Data
Sandwich ELISA - Mouse H-FABP ELISA Kit (AB242240)
Interpolated concentrations of native H-FABP in mouse heart extract and skeletal muscle extract based on 100 ng/mL and 250 ng/mL extract loads, respectively.
The concentrations of H-FABP were measured in duplicate and interpolated from the H-FABP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP3 concentration was determined to be 427.5 pg/mL in heart extract and 344.3 pg/mL in skeletal muscle extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse H-FABP ELISA Kit (AB242240)
Example of mouse H-FABP standard curve in 1X Cell Extraction Buffer PTR.
The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse H-FABP ELISA Kit (ab242240) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of H-FABP protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse H-FABP with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Fatty acid binding largely defines H-FABP's role facilitating the transport of fatty acids within cells which is key for energy production. It is not part of a larger protein complex but plays into broader lipid metabolism processes. The efficiency of H-FABP in fatty acid binding and transport helps maintain cellular energy homeostasis especially in tissues with high fatty acid metabolism like the heart.
Pathways
H-FABP integrates into the fatty acid metabolic pathways and the beta-oxidation pathway. It is associated with the transport and utilization of fatty acids linking it to the peroxisome proliferator-activated receptor (PPAR) signaling pathway an important regulator of lipid metabolism. Proteins such as CD36 and PPARα interact with H-FABP within these pathways influencing lipid storage and energy balance.
Product protocols
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Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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