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AB157714

Mouse Haptoglobin ELISA Kit

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(7 Publications)

Mouse Haptoglobin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Haptoglobin in Mouse in Biofluids samples.
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Sandwich ELISA - Mouse Haptoglobin ELISA Kit (AB157714)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Haptoglobin ELISA Kit (AB157714)

Representative standard curve using ab157714 Haptoglobin Mouse ELISA kit

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.484 ng/mL

Range

1.95 - 125 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Haptoglobin ELISA kit is designed for the quantitative measurement of Haptoglobin in mouse serum, plasma and other biological samples.

In this assay the Haptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Haptoglobin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Haptoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Haptoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Haptoglobin in the test sample. The quantity of Haptoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157714-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157714-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157714-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mouse Haptoglobin ELISA Microplate", "number":"AB157714-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Haptoglobin Calibrator (lyophilized)", "number":"AB157714-CMP04", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-mouse Haptoglobin antibody", "number":"AB157714-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157714-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157714-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.
Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:3953 PubMed36894646

2023

Decreased Paneth cell α-defensins promote fibrosis in a choline-deficient L-amino acid-defined high-fat diet-induced mouse model of nonalcoholic steatohepatitis via disrupting intestinal microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Shunta Nakamura,Kiminori Nakamura,Yuki Yokoi,Yu Shimizu,Shuya Ohira,Mizu Hagiwara,Zihao Song,Li Gan,Tomoyasu Aizawa,Daigo Hashimoto,Takanori Teshima,Andre J Ouellette,Tokiyoshi Ayabe

Science advances 8:eabq4469 PubMed36197975

2022

Iron regulatory protein (IRP)-mediated iron homeostasis is critical for neutrophil development and differentiation in the bone marrow.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Bonadonna,Sandro Altamura,Elisabeth Tybl,Gael Palais,Maria Qatato,Maria Polycarpou-Schwarz,Martin Schneider,Christina Kalk,Wibke Rüdiger,Alina Ertl,Natasha Anstee,Ruzhica Bogeska,Dominic Helm,Michael D Milsom,Bruno Galy

Physiological reports 9:e14844 PubMed34042301

2021

Early life stress induces dysregulation of the heme pathway in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yasminye D Pettway,Thomas H Neder,Dao H Ho,Brandon M Fox,Mariah Burch,Jackson Colson,Xiaofen Liu,Cailin E Kellum,Kelly A Hyndman,Jennifer S Pollock

Advanced therapeutics 4: PubMed34095458

2021

Synthetic platelet microgels containing fibrin knob B mimetic motifs enhance clotting responses.

Applications

Unspecified application

Species

Unspecified reactive species

Seema Nandi,Emily Mihalko,Kimberly Nellenbach,Mario Castaneda,John Schneible,Mary Harp,Halston Deal,Michael Daniele,Stefano Menegatti,Thomas H Barker,Ashley C Brown

Cell host & microbe 24:271-284.e3 PubMed30033122

2018

α-Toxin Induces Platelet Aggregation and Liver Injury during Staphylococcus aureus Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Bas G J Surewaard,Ajitha Thanabalasuriar,Zhutian Zeng,Christine Tkaczyk,Taylor S Cohen,Bart W Bardoel,Selina K Jorch,Carsten Deppermann,Juliane Bubeck Wardenburg,Rachelle P Davis,Craig N Jenne,Kendall C Stover,Bret R Sellman,Paul Kubes

Pharmacological research 134:157-165 PubMed29909116

2018

Altered microRNA regulation of short chain fatty acid receptors in the hypertensive kidney is normalized with hydrogen sulfide supplementation.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory J Weber,Jaleyea Foster,Sathnur B Pushpakumar,Utpal Sen

International journal of molecular medicine 39:1357-1370 PubMed28440464

2017

Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy.

Applications

ELISA

Species

Unspecified reactive species

Sandra Murphy,Paul Dowling,Margit Zweyer,Michael Henry,Paula Meleady,Rustam R Mundegar,Dieter Swandulla,Kay Ohlendieck
View all publications
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