AB282873

Mouse HDAC8 ELISA Kit

Recombinant
SimpleStep
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Mouse HDAC8 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse HDAC8 with a sensitivity of 13 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8, Hdac8

2 Images

Sandwich ELISA - Mouse HDAC8 ELISA Kit (AB282873)

sELISA

Supplier Data

Sandwich ELISA - Mouse HDAC8 ELISA Kit (AB282873)

Example of mouse HDAC8 standard curve in 1X Cell Extraction Buffer PTR

sELISA

Supplier Data

Sandwich ELISA - Mouse HDAC8 ELISA Kit (AB282873)

Interpolated concentrations of native HDAC8 in mouse NIH/3T3 cells based on a 1,000 μg/mL extract load.

The concentrations of HDAC8 were measured in duplicate and interpolated from the HDAC8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HDAC8 concentration was determined to be 4,606 pg/mL in NIH/3T3 cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 13 pg/mL

Range

140.63 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse HDAC8 ELISA kit (ab282873) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse HDAC8 protein in mouse cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HDAC8 with 13 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

HDAC8 (Histone deacetylase 8) is a nuclear enzyme responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 is also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. Mouse HDAC8 shares 99.7% amino acid sequence identity with rat HDAC8. Human HDAC8 is 96% identical to mouse HDAC8.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "92 - 118 %", "average": "= 104" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282873-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282873-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282873-CMP14", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB282873-CMP15", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282873-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HDAC8 Capture Antibody", "number":"AB282873-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse HDAC8 Lyophilized Recombinant Protein", "number":"AB282873-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB282873-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282873-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282873-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282873-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282873-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HDAC8 Detector Antibody", "number":"AB282873-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.

Biological function summary

HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.

Pathways

Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.

Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.

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Product protocols

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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones.

See full target information Hdac8

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse HDAC8 ELISA Kit

Sandwich ELISA - Mouse HDAC8 ELISA Kit (AB282873)

Sandwich ELISA - Mouse HDAC8 ELISA Kit (AB282873)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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