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AB245724

Mouse HE4 ELISA Kit

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Mouse HE4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse HE4 with a sensitivity of 1.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

He4, Wfdc2, WAP four-disulfide core domain protein 2, WAP domain-containing protein HE4

1 Images
Sandwich ELISA - Mouse HE4 ELISA Kit (AB245724)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse HE4 ELISA Kit (AB245724)

Example of mouse HE4 standard curve in Sample Diluent NS. The HE4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.99 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse HE4 ELISA Kit (ab245724) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HE4 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HE4 with 1.99 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245724-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245724-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245724-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB245724-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245724-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245724-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245724-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HE4 Detector Antibody", "number":"AB245724-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245724-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HE4 Capture Antibody", "number":"AB245724-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse HE4 Lyophilized Recombinant Protein", "number":"AB245724-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human Epididymis Protein 4 (HE4) also known as WAP four-disulfide core domain protein 2 (WFDC2) is a small glycoprotein of approximately 13 kDa. HE4 is most highly expressed in the epithelial cells of reproductive tissues like the epididymis but also in tissues such as the respiratory and urogenital tracts. HE4 is secreted into the extracellular space where it functions and is found in normal range in different body fluids. This secreted protein has gained attention due to its use in various diagnostic tests such as the HE4 test and HE4 assay utilizing HE4 antibodies.
Biological function summary

HE4 participates in innate immune response and epithelial cell protection. HE4 protein plays a role in the modulation of antiprotease activity as it is involved in inhibiting serine proteases. It is not part of a larger protein complex but interacts with multiple protease signaling pathways. HE4 is often studied for its role in modulating protein activity at epithelial barriers contributing to tissue homeostasis and protective mechanisms against infections.

Pathways

HE4 interacts within signaling cascades such as the innate immune pathways and extracellular matrix organization. It links to proteins within these pathways like serine proteases and other WFDC family proteins. HE4 influences protease activity regulation an essential component of tissue remodeling and inflammatory response impacting overall pathway dynamics.

HE4 has strong associations with ovarian cancer and endometrial cancer. Its overexpression in these malignancies makes it a valuable biomarker for disease diagnosis and prognosis. HE4 interacts with cancer-related proteins such as CA125 enhancing its utility in combinatory diagnostic approaches. The HE4 test and HE4 ELISA kit price underline its significance in clinical settings for monitoring these cancers where timely detection influences treatment outcomes.
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Product protocols

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Target data

Broad range protease inhibitor.
See full target information Wfdc2
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websiteProtocolBooklet
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Product promise

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