AB204524

Mouse Heme Oxygenase 1 ELISA Kit

Recombinant
SimpleStep
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(16 Publications)

Mouse Heme Oxygenase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Heme Oxygenase 1 with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Heme oxygenase 1, HO-1, P32 protein, Hmox1

7 Images

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Example of Heme Oxygenase 1 standard curve diluted in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Interpolated concentrations of Heme Oxygenase 1 in mouse plasma (EDTA), plasma (heparin) and plasma (citrate).

The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 5,536 pg/mL in mouse plasma (EDTA), 6,400 pg/mL in mouse plasma (heparin), and 5,479 pg/mL in mouse plasma (Citrate).

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Interpolated concentrations of Heme Oxygenase 1 in mouse serum, and splenocyte supernatant.

The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 10,670 pg/mL in mouse serum and 6,084 pg/mL in mouse splenocyte supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Quantitation of Heme Oxygenase 1 expression in different cell extracts.

Interpolated values of Heme Oxygenase 1 are plotted for the indicated cell extracts (y-axis) against the total extract load (x-axis).

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Example of Heme Oxygenase 1 standard curve diluted in Sample Diluent NS. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Example of Heme Oxygenase 1 standard curve diluted in 1X Cell Extraction Buffer PTR. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Example of Heme Oxygenase 1 standard curve diluted in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10 pg/mL

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Heme Oxygenase 1 ELISA Kit (ab204524) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heme Oxygenase 1 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, urine, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Heme Oxygenase 1 with 10 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant mouse Heme Oxygenase 1 protein in serum, plasma, urine, cell culture supernatant, cell and tissue extract samples only.

SPECIES REACTIVITY

This kit recognizes mouse Heme Oxyenase 1 protein.

Other species reactivity was determined by measuring 4-fold diluted serum samples of various species, interpolating the protein concentrations from the mouse standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in mouse serum assayed at the same dilution.

Reactivity < 3% was determined for the following species: Human, Guinea Pig, Rabbit, Dog, Pig, Cow

Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 47% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "94 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "87 - 91 %", "average": "= 89" }, { "sample": "Urine", "range": "102 - 111 %", "average": "= 105" }, { "sample": "Tissue Extracts", "range": "104 - 113 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "87 - 108 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "108 - 119 %", "average": "= 112" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab204524-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB204524-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB204524-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB204524-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB204524-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB204524-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB204524-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB204524-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HO 1 Capture Antibody", "number":"AB204524-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse HO 1 Lyophilized Recombinant Protein", "number":"AB204524-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB204524-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HO 1 Detector Antibody", "number":"AB204524-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB204524-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme oxygenase 1 (HO-1) also known as heme oxygenase-1 or oxygenase catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. It has been characterized with a mass of approximately 32 kDa. HO-1 is widely expressed in tissues with high metabolic activity such as liver spleen bone marrow and brain. This enzyme plays key roles in cellular response to oxidative stress and is responsive to various stress-inducing stimuli.

Biological function summary

The function of HO-1 involves important antioxidant defense mechanisms. The enzyme contributes to the cytoprotective effects by moderating oxidative stress and inflammation through its enzymatic activity. HO-1 often forms part of a larger regulatory complex where its activity regulates other cellular processes. Its heme byproducts biliverdin and bilirubin possess strong antioxidant properties adding to the cell's capacity to neutralize harmful oxidative effects.

Pathways

HO-1 operates in heme degradation and the antioxidative response pathway. It closely interacts with ferritin in its role of managing intracellular iron levels. As HO-1 breaks down heme the free iron released is sequestered by ferritin preventing cellular iron overload. HO-1 also plays a part in the signaling pathway involved in maintaining vascular homeostasis where it may modulate the activity of nitric oxide synthase through its byproducts.

Dysregulation of HO-1 levels connects to conditions such as cardiovascular diseases and certain neurodegenerative disorders. In cardiovascular diseases especially atherosclerosis the improper balance of heme oxygenase-1 activity can influence the severity of oxidative damage. Furthermore HO-1 relates to the pathogenesis of Alzheimer's disease where oxidative stress plays a significant role and where its interaction with proteins like amyloid-beta if disrupted may worsen disease pathology.

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Product protocols

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Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (By similarity) Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (By similarity).. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information Hmox1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:746 PubMed40360769

2025

Malnutrition exacerbates pathogenesis of Lutzomyia longipalpis sand fly-transmitted Leishmania donovani.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Iniguez,Johannes Doehl,Pedro Cecilio,Tiago Donatelli Serafim,Caroline Percopo,Yvonne Rangel-Gonzalez,Somaditya Dey,Elvia J Osorio,Patrick Huffcutt,Sofia Roitman,Claudio Meneses,Mara Short,Jesus G Valenzuela,Peter C Melby,Shaden Kamhawi

Cellular and molecular neurobiology 45:13 PubMed39833644

2025

Aging and MPTP Sensitivity Depend on Molecular and Ultrastructural Signatures of Astroglia and Microglia in Mice Substantia Nigra.

Applications

Unspecified application

Species

Unspecified reactive species

P L Abhilash,Upasna Bharti,Santhosh Kumar Rashmi,Mariamma Philip,T R Raju,Bindu M Kutty,B K Chandrasekhar Sagar,Phalguni Anand Alladi

International journal of molecular sciences 23: PubMed35008897

2021

Dysregulated Autophagy and Mitophagy in a Mouse Model of Duchenne Muscular Dystrophy Remain Unchanged Following Heme Oxygenase-1 Knockout.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Mucha,Katarzyna Kaziród,Paulina Podkalicka,Kinga Rusin,Józef Dulak,Agnieszka Łoboda

Pharmaceutical biology 59:114-120 PubMed33550883

2021

Eucalyptol ameliorates early brain injury after subarachnoid haemorrhage via antioxidant and anti-inflammatory effects in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Xu,Junsheng Guo,Chunming Sun

Frontiers in molecular neuroscience 13:84 PubMed32625061

2020

Metformin Protects From Rotenone-Induced Nigrostriatal Neuronal Death in Adult Mice by Activating AMPK-FOXO3 Signaling and Mitigation of Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sabah H El-Ghaiesh,Hoda I Bahr,Afaf T Ibrahiem,Doaa Ghorab,Suliman Y Alomar,Noha E Farag,Sawsan A Zaitone

Nature communications 11:2788 PubMed32493916

2020

Ultrasmall copper-based nanoparticles for reactive oxygen species scavenging and alleviation of inflammation related diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Liu,Bowen Xiao,Fei Xiang,Jianglin Tan,Zhuo Chen,Xiaorong Zhang,Chengzhou Wu,Zhengwei Mao,Gaoxing Luo,Xiaoyuan Chen,Jun Deng

Circulation reports 2:60-68 PubMed33693175

2019

5-Aminolevulinic Acid Attenuates Atherosclerotic Plaque Progression in Low-Density Lipoprotein Receptor-Deficient Mice by Heme Oxygenase-1 Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Kohsuke Hagisawa,Makoto Ayaori,Katsunori Ikewaki,Motowo Nakajima,Yuji Morimoto

Brain research bulletin 154:21-31 PubMed31589901

2019

Ac-SDKP ameliorates the progression of experimental autoimmune encephalomyelitis via inhibition of ER stress and oxidative stress in the hippocampus of C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Pejman,Maryam Kamarehei,Gholamhossein Riazi,Shahriar Pooyan,Saeed Balalaie

Phytotherapy research : PTR 33:3163-3176 PubMed31468630

2019

Panax notoginseng saponins suppress lipopolysaccharide-induced barrier disruption and monocyte adhesion on bEnd.3 cells via the opposite modulation of Nrf2 antioxidant and NF-κB inflammatory pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shaonan Hu,Tingting Liu,Yali Wu,Wanqing Yang,Shaobo Hu,Zongxi Sun,Pengyue Li,Shouying Du

Cytokine 113:417-426 PubMed30539784

2018

The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination.

Applications

ELISA

Species

Mouse

Hamid Zahednasab,Masoumeh Firouzi,Sussan Kaboudanian-Ardestani,Zahra Mojallal-Tabatabaei,Sajad Karampour,Hossein Keyvani

View all publications

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Mouse Heme Oxygenase 1 ELISA Kit

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit (AB204524)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise