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AB285317

Mouse Hemoglobin A1c ELISA Kit

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(2 Publications)

Mouse Hemoglobin A1c ELISA Kit is a sandwich ELISA designed to quantify Mouse Hemoglobin A1c with a sensitivity of 0.75 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.25 - 80 ng/mL
1 Images
Sandwich ELISA - Mouse Hemoglobin A1c ELISA Kit (AB285317)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Hemoglobin A1c ELISA Kit (AB285317)

Mouse Hemoglobin A1c ELISA Kit - Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Tissue Homogenate, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.75 ng/mL

Range

1.25 - 80 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Hemoglobin A1c ELISA Kit ab285317 is a sandwich ELISA to measure Mouse Hemoglobin A1c in cell culture supernatant, tissue homogenate, other biological fluids with a sensitivity of 0.75 ng/ml.

How the assay works

The density of color is proportional to the amount of HbA1c captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Hemoglobin A1c ELISA Kit ab285317 protocol summary

1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin A1c also known as HbA1c or glycohemoglobin is a form of hemoglobin that is chemically linked to a glucose molecule. It has a molecular weight of approximately 64 kDa. This protein is primarily found in red blood cells where it carries oxygen from the lungs to the rest of the body. Hemoglobin A1c is produced when glucose in the blood binds irreversibly to hemoglobin a process known as glycation. Researchers measure Hemoglobin A1c levels to manage blood sugar control particularly in diabetic patients.
Biological function summary

Hemoglobin A1c is a marker for assessing long-term glycemic control. It does not operate as part of a larger complex but modifies the hemoglobin protein inside erythrocytes. The level of HbA1c reflects the average blood glucose concentration over the previous two to three months. Its presence and levels offer insight into the overall metabolic state providing important information for clinicians while managing conditions like diabetes. HbA1c is important since it allows monitoring without the need for daily blood sugar measurements.

Pathways

HbA1c integrates into the glucose metabolism pathway and is linked to protein glycation pathways. Within the glucose metabolism pathway glycated hemoglobin serves as a longitudinal biomarker. It does not directly interact with other proteins like insulin but insulin levels can indirectly influence the HbA1c levels by regulating blood glucose levels. The accurate measurement of HbA1c by HbA1c assay or HbA1c ELISA provides insights into the efficacy of diabetes management strategies.

Hemoglobin A1c plays a significant role in diabetes mellitus and cardiovascular disease. High levels of HbA1c indicate poor glycemic control which is associated with complications of diabetes such as retinopathy. In cardiovascular disease elevated HbA1c levels act as a risk factor for adverse outcomes. The relationship between HbA1c and these diseases also highlights connections with other proteins such as insulin which regulates glucose and subsequently HbA1c levels. Utilizing HA1c test kits and HbA1c kits healthcare providers can monitor potential long-term complications in patients with elevated HbA1c levels.
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 25:151 PubMed40269867

2025

Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jialuo Cai,Yilin Zhu,Guiming Deng,Linqi Ouyang,Wangzhong Xiao,Fang Zhou,Yuanshan Han,Feiyun Yuan,Li Huang,Xiaoping Li

International journal of surgery (London, England) 111:273-282 PubMed38935106

2024

Semaglutide outperforms insulin in restoring neutrophil function against implant-related infection in diabetic and obese mice: experimental research.

Applications

Unspecified application

Species

Unspecified reactive species

Tiexin Liu,Lenian Zhou,Yiwei Chen,Junqing Lin,Hongyi Zhu
View all publications
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websiteProtocolBooklet
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Product promise

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