Mouse Hemoglobin ELISA Kit

Product details

Mouse Hemoglobin ELISA Kit ab157715 is a sandwich ELISA to measure Mouse Hemoglobin in serum, plasma with a sensitivity of 2.62 ng/ml.

How the assay works

In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-HM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound HM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HM in the test sample. The quantity of HM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Hemoglobin ELISA Kit ab157715 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development