AB324324

Mouse Heparanase 1 ELISA Kit

SimpleStep
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Mouse Heparanase 1 ELISA Kit is a Sandwich ELISA for the measurement of Mouse Heparanase 1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Hpa, Heparanase, Endo-glucoronidase

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Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Interpolated concentration of native Heparanase 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Example of mouse Heparanase 1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Interpolated concentration of native Heparanase 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 2%, plasma citrate 25%, plasma EDTA 25%, and spleen supernatant 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Interpolated concentration of native Heparanase 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : mouse serum 2% and rat serum 8%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Heparanase 1 ELISA Kit (AB324324)

Example of mouse Heparanase 1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 30.43 pg/mL

Range

65.625 - 4200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Heparanase 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heparanase 1 protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Mouse Heparanase 1 with 30.43 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.2" } ]

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Recovery

[ { "sample": "Serum", "range": "101 - 102 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "84 - 96 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "92 - 101 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "94 - 96 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

See full target information Hpse

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websiteProtocolBooklet

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Product promise

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