AB285280

Mouse Hepcidin ELISA Kit

Name: Mouse Hepcidin ELISA Kit (ab285280) | Abcam
Brand: Abcam Limited
SKU: AB285280
Price: 960 USD
Availability: InStock

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(1 Publication)

Mouse Hepcidin ELISA Kit is a sandwich ELISA designed to quantify Mouse Hepcidin with a sensitivity of 1.875 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 3.125 - 200 ng/mL

View Alternative Names

Hamp1, Hepc, Hepc1, Hamp, Hepcidin

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Sandwich ELISA - Mouse Hepcidin ELISA Kit (AB285280)

sELISA

Supplier Data

Sandwich ELISA - Mouse Hepcidin ELISA Kit (AB285280)

Mouse Hepcidin ELISA Kit - Standard Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 1.875 ng/mL

Range

3.125 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Hepcidin ELISA Kit ab285280 is a sandwich ELISA to measure Mouse Hepcidin in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 1.875 ng/ml.

How the assay works

The density of color is proportional to the amount of Hepcidin captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Hepcidin ELISA Kit ab285280 protocol summary

1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4693 Hepcidin (mouse) ELISA Kit. E4693-100 is the same size as the 96 test size of ab285280.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285280-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285280-CMP03", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285280-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285280-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285280-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285280-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285280-CMP11", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285280-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285280-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin-Detection antibody (Concentrated)", "number":"AB285280-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285280-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285280-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin also known as Liver-Expressed Antimicrobial Peptide (LEAP) or HEPC is an important regulator of iron homeostasis functioning as a hormone. It consists of a small peptide typically around 25 amino acids in length with a molecular mass of approximately 2.8 kDa. Hepcidin is produced predominantly in the liver and is secreted into the bloodstream where it directly binds to the iron export protein ferroportin. This binding induces ferroportin's internalization and degradation effectively restricting iron release into the circulation and controlling systemic iron levels.

Biological function summary

This hormone plays a critical role in maintaining iron balance within the body by regulating both the absorption of dietary iron in the intestine and the release of stored iron from macrophages and hepatocytes. Hepcidin operates independently and is not part of any larger complex. Its levels fluctuate in response to various stimuli including iron levels inflammation and erythropoietic demand impacting iron transport and storage.

Pathways

Hepcidin is integral to the iron metabolism pathway. It interacts intricately with proteins such as transferrin and ferritin which are involved in iron transport and storage. Hepcidin's function is modulated by the BMP/SMAD signaling pathway as well where its expression is promoted by these signaling proteins. This regulation ensures that iron levels are properly maintained for critical processes such as hemoglobin synthesis and cellular respiration.

Hepcidin is connected to conditions such as anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease increased hepcidin levels lead to iron sequestration and restricted availability which contributes to the anemic state. In hereditary hemochromatosis mutations can result in decreased hepcidin activity causing excessive iron accumulation in the body. Through these mechanisms hepcidin's interactions with proteins such as ferroportin and transferrin receptor 2 influence the development and progression of these iron-related disorders.

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Product protocols

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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

See full target information Hamp

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JHEP reports : innovation in hepatology 5:100695 PubMed36968217

2023

TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Wantong Su,Weicheng Gao,Rui Zhang,Qi Wang,Lei Li,Qingfa Bu,Zibo Xu,Zheng Liu,Mingming Wang,Yaqing Zhu,Guoping Wu,Haoming Zhou,Xun Wang,Ling Lu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Hepcidin ELISA Kit

Sandwich ELISA - Mouse Hepcidin ELISA Kit (AB285280)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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