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AB100686

Mouse HGF ELISA Kit

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(2 Publications)

Mouse HGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse HGF in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hgf

2 Images
Sandwich ELISA - Mouse HGF ELISA Kit (AB100686)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse HGF ELISA Kit (AB100686)

Representative Standard Curve using ab100686

Sandwich ELISA - Mouse HGF ELISA Kit (AB100686)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse HGF ELISA Kit (AB100686)

Representative Standard Curve using ab100686

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 0.4 ng/mL

Range

0.614 - 60 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 123 %", "average": "= 111.8" }, { "sample": "Serum", "range": "98 - 129 %", "average": "= 111.4" }, { "sample": "Plasma", "range": "80 - 120 %", "average": "= 105.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100686-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100686-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100686-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100686-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100686-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse HGF", "number":"AB100686-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HGF Microplate (12 x 8 wells)", "number":"AB100686-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100686-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse HGF Standard (lyophilized)", "number":"AB100686-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100686-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
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Product protocols

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Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). Activates MAPK signaling following TMPRSS13 cleavage and activation (By similarity).
See full target information Hgf
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed31238604

2019

Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Boldyreva,Evgeny K Shevchenko,Yuliya D Molokotina,Pavel I Makarevich,Irina B Beloglazova,Ekaterina S Zubkova,Konstantin V Dergilev,Zoya I Tsokolaeva,Dmitry Penkov,Mu-Nung Hsu,Yu-Chen Hu,Yelena V Parfyonova

Cell reports 3:401-10 PubMed23375376

2013

Liver-derived systemic factors drive β cell hyperplasia in insulin-resistant states.

Applications

Unspecified application

Species

Unspecified reactive species

Abdelfattah El Ouaamari,Dan Kawamori,Ercument Dirice,Chong Wee Liew,Jennifer L Shadrach,Jiang Hu,Hitoshi Katsuta,Jennifer Hollister-Lock,Wei-Jun Qian,Amy J Wagers,Rohit N Kulkarni
View all publications
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websiteProtocolBooklet
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