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AB100687

Mouse HGF ELISA Kit

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(6 Publications)

Mouse HGF ELISA Kit is a Sandwich ELISA for the measurement of Mouse HGF in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hgf

1 Images
Sandwich ELISA - Mouse HGF ELISA Kit (AB100687)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse HGF ELISA Kit (AB100687)

Representative Standard Curve using ab100687

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 100 pg/mL

Range

0.25 - 60 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse HGF in cell lysates and tissue lysates.

This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "100 - 134 %", "average": "= 114.4" }, { "sample": "Tissue Extracts", "range": "110 - 130 %", "average": "= 120.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100687-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100687-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HGF Microplate (12 x 8 wells)", "number":"AB100687-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100687-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100687-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100687-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100687-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100687-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse HGF Standard (lyophilized)", "number":"AB100687-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse HGF", "number":"AB100687-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100687-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
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Product protocols

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Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). Activates MAPK signaling following TMPRSS13 cleavage and activation (By similarity).
See full target information Hgf
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Gastrointestinal and liver physiology 321:G378-G388 PubMed34405729

2021

Nrf2 expression in pancreatic stellate cells promotes progression of cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tanaka,Shin Hamada,Ryotaro Matsumoto,Keiko Taguchi,Masayuki Yamamoto,Atsushi Masamune

Experimental and therapeutic medicine 15:3096-3102 PubMed29599843

2018

Platelet-rich plasma shows beneficial effects for patients with knee osteoarthritis by suppressing inflammatory factors.

Applications

Unspecified application

Species

Unspecified reactive species

Guilin Huang,Sha Hua,Tuanmin Yang,Jianbing Ma,Wenxing Yu,Xiujin Chen

Cell death & disease 8:e2811 PubMed28542142

2017

Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Li,Chentong Xu,Xin Lin,Liujing Qu,Dan Xia,Beiqi Hongdu,Yan Xia,Xiaokun Wang,Yaxin Lou,Qihua He,Dalong Ma,Yingyu Chen

Laboratory investigation; a journal of technical methods and pathology 93:181-93 PubMed23212100

2012

The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Takehara,Akiko Yabuuchi,Kenji Ezoe,Tomoko Kuroda,Rie Yamadera,Chiaki Sano,Nana Murata,Takuya Aida,Ken Nakama,Fumihito Aono,Naoki Aoyama,Keiich Kato,Osamu Kato

Endocrinology 153:5760-9 PubMed23024263

2012

Hepatocyte growth factor plays a key role in insulin resistance-associated compensatory mechanisms.

Applications

Unspecified application

Species

Rat

Tiago G Araújo,Alexandre G Oliveira,Bruno M Carvalho,Dioze Guadagnini,André O P Protzek,Jose B C Carvalheira,Antonio C Boschero,Mario J A Saad

Diabetes 61:1143-52 PubMed22427375

2012

Loss of HGF/c-Met signaling in pancreatic β-cells leads to incomplete maternal β-cell adaptation and gestational diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Cem Demirci,Sara Ernst,Juan C Alvarez-Perez,Taylor Rosa,Shelley Valle,Varsha Shridhar,Gabriella P Casinelli,Laura C Alonso,Rupangi C Vasavada,Adolfo García-Ocana
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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