Mouse HGF ELISA Kit
- Recombinant
- SimpleStep
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(3 Publications)
Mouse HGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse HGF with a sensitivity of 8.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hgf
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Recombinant rat and mouse HGF standard curves.
Recombinant rat HGF was prepared at 4 ng/mL and diluted with a 2-fold titration alongside of mouse HGF standard curve, and assayed for cross reactivity. 100% cross-reactivity was observed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Interpolated concentrations of native HGF in mouse serum and plasma samples.
The concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 11,764 pg/mL in serum, 1099 pg/mL in plasma (EDTA), 691 pg/mL in plasma (citrate) and 896 pg/mL in plasma heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Interpolated concentrations of native HGF in rat serum and plasma samples.
The concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (EDTA) 100%, plasma (citrate) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 79,543 pg/mL in serum, 3,151 pg/mL in plasma (EDTA), 2,395 pg/mL in plasma (citrate) and 2,611 pg/mL in plasma heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Interpolated concentrations of native HGF in mouse cell and tissue extracts, extracts based on a 4,000 μg/mL extract load.
The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 7,859 pg/mL in NIH/3T3 cell extract, 2,472 pg/mL in mouse spleen tissue extract and 1,291 pg/mL in mouse kidney tissue extract.
- sELISA
Lab
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Mouse HGF standard curve comparison data.
Standard curve comparison between mouse HGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Interpolated concentrations of native HGF in mouse 3T3-L1 undifferentiated and 3T3-L1 adipocyte differentiated cell extract samples, sample based on a 1,000 μg/mL extract load.
Mouse 3T3-L1 cells were either undifferentiated (not allowed to reach confluency) or differentiated (cultured for 48 hours in adipocyte differentiation medium). The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 2,636 pg/mL in undifferentiated 3T3-L1 cell extract and 88 pg/mL in adipocyte differentiated 3T3-L1 cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Example of mouse HGF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)
Example of mouse HGF standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse HGF ELISA Kit (ab223862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HGF protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HGF with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Hepatocyte Growth Factor (HGF) is mitogen of parenchymal hepatocytes. HGF also acts as a growth factor and is expressed by a wide variety of tissue and cell types. HGF binds to the HGF- Receptor to help regulate cell growth, cell motility and morphogenesis. Elevated circulating levels of HGF can be measured as prognostic marker of severity of hypertension and heart failure. HGF is secreted as a full length inactive polypeptide, and then is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta chain which dimerize producing the active heterodimeric molecule. Mouse HGF SimpleStep ELISA® (ab223862) is designed to recognize this dimerized protein.
The standard is affinity purified.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Recovery
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Properties and storage information
Shipped at conditions
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.
Pathways
HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.
Product protocols
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Publications (3)
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International journal of molecular sciences 25: PubMed38256259
2024
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Stem cell research & therapy 13:279 PubMed35765096
2022
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Military Medical Research 8:16 PubMed33622404
2021
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