Mouse HIF-1 alpha ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(1 Publication)
Mouse HIF-1 alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse HIF-1 alpha with a sensitivity of 33.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Hif1a
- sELISA
Supplier Data
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit (AB275103)
Example of mouse HIF-1alpha standard curve in 1X Cell Extraction Buffer PTR.
The HIF-1alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit (AB275103)
Interpolated concentrations of native HIF-1alpha in mouse deferoxamine treated NIH/3T3 cell extract based on a 10 μg/mL extract load.
The concentrations of HIF-1alpha were measured in duplicate, interpolated from the HIF-1alpha standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF-1alpha concentration was determined to be 485.55 pg/mL and 40.77 pg/ml in deferoxamine and mock treated cell extract, respectively.
- sELISA
Supplier Data
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit (AB275103)
Interpolated concentrations of native HIF-1alpha in mouse NIH/3T3 cell extract either treated with deferoxamine or mock treatment based on a 100 μg/mL extract load.
The concentrations of HIF-1alpha were measured in duplicate, interpolated from the HIF-1alpha standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF-1alpha concentration was determined to be 1481 pg/mL.
Complementary Products
Reactivity data
Product details
Mouse HIF-1 alpha ELISA kit (ab275103) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse HIF-1 alpha protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HIF-1 alpha with 33.39 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Hypoxia-inducible factor 1-alpha (HIF-1 alpha) is a constitutively expressed transcription factor that is degraded under normal oxygen tensions and stabilized when oxygen is limiting (hypoxia). Under hypoxic conditions, stabilized HIF-1 alpha translocates to the nucleus and promotes the transcription of genes that enable the cell to adapt to a low oxygen environment. The HIF-1 alpha mediated hypoxic response include promotion of angiogenesis and the switch from aerobic respiration to anaerobic glycolysis. Many of the HIF-1 alpha responsive genes encode proteins that promote glycolysis and/or inhibit oxidative phosphorylation included in the Warburg effect.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in pharmacology 12:720173 PubMed35095479
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com