Mouse HIF-1 alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse HIF-1 alpha with a sensitivity of 33.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in pharmacology 12:7201732022AKT-AMPKα-mTOR-dependent HIF-1α Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEslam E Abd El-Fattah,Sameh Saber,Mahmoud E Youssef,Hanan Eissa,Eman El-Ahwany,Noha A Amin,Mohammed Alqarni,Gaber El-Saber Batiha,Ahmad J Obaidullah,Mohamed M Y Kaddah,Ahmed Gaafar Ahmed Gaafar,Ahmed A E Mourad,Gomaa Mostafa-Hedeab,Amir Mohamed AbdelhamidPubMed 35095479
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 117.19 - 7500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 33.39 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:15225651, PubMed:17981124, PubMed:22009797). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).
Alternative names
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Hif1a
What's included?
Recommended products
Mouse HIF-1 alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse HIF-1 alpha with a sensitivity of 33.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 117.19 - 7500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 33.39 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 15 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 96 | Range 87 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse HIF-1 alpha ELISA kit (ab275103) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse HIF-1 alpha protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse HIF-1 alpha with 33.39 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Hypoxia-inducible factor 1-alpha (HIF-1 alpha) is a constitutively expressed transcription factor that is degraded under normal oxygen tensions and stabilized when oxygen is limiting (hypoxia). Under hypoxic conditions, stabilized HIF-1 alpha translocates to the nucleus and promotes the transcription of genes that enable the cell to adapt to a low oxygen environment. The HIF-1 alpha mediated hypoxic response include promotion of angiogenesis and the switch from aerobic respiration to anaerobic glycolysis. Many of the HIF-1 alpha responsive genes encode proteins that promote glycolysis and/or inhibit oxidative phosphorylation included in the Warburg effect.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
- Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
- Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
- Associated diseases and disorders
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
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3 product images
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit (ab275103)
Example of mouse HIF-1alpha standard curve in 1X Cell Extraction Buffer PTR.
The HIF-1alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit (ab275103)
Interpolated concentrations of native HIF-1alpha in mouse NIH/3T3 cell extract either treated with deferoxamine or mock treatment based on a 100 μg/mL extract load.
The concentrations of HIF-1alpha were measured in duplicate, interpolated from the HIF-1alpha standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF-1alpha concentration was determined to be 1481 pg/mL.
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit (ab275103)
Interpolated concentrations of native HIF-1alpha in mouse deferoxamine treated NIH/3T3 cell extract based on a 10 μg/mL extract load.
The concentrations of HIF-1alpha were measured in duplicate, interpolated from the HIF-1alpha standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF-1alpha concentration was determined to be 485.55 pg/mL and 40.77 pg/ml in deferoxamine and mock treated cell extract, respectively.
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