JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325897

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse HIF-1 alpha with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 10.29 - 22500 pg/ml with a sensitivity of 2.87 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Hif1a

5 Images
Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)
  • sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Standard curve comparison between the Chemiluminescent Mouse HIF-1 alpha SimpleStep ELISA and colorimetric Mouse HIF-1 alpha SimpleStep ELISA (ab275103) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)
  • sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Interpolated concentrations of native HIF-1 alpha in mouse deferoxamine treated versus untreated NIH/3T3 cell extract based on a 10 µg/mL extract load. The concentrations of HIF1a were measured in duplicate, interpolated from the HIF1a standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF1a concentration was determined to be 485.55 pg/mL and 40.77 pg/ml in deferoxamine and mock treated cell extract, respectively.

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)
  • sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Interpolated concentrations of native HIF-1 alpha in mouse NIH/3T3 cell extract treated with deferoxamine based on a 100 µg/mL extract load. The concentrations of HIF1a were measured in duplicate, interpolated from the HIF1a standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF1a concentration was determined to be 1,481 pg/mL.

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)
  • sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

Dynamic range comparison between the Chemiluminescent Mouse HIF-1 alpha SimpleStep ELISA, colorimetric Mouse HIF-1 alpha SimpleStep ELISA (ab275103), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)
  • sELISA

Lab

Sandwich ELISA - Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (AB325897)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Luminescent

Sample types

Cell Extract, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.87 pg/mL

Range

10.29 - 22500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (ab325897) is a rapid single-wash sandwich 55-min ELISA to measure Mouse HIF-1 alpha in Cell Extract, Tissue Extract with a sensitivity of 2.87 pg/mL.

How the assay works

Mouse HIF-1 alpha ELISA Kit, Chemiluminescent (ab325897) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse HIF-1 alpha (ab325897) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Mouse-Hif1a antibody [EPR23071-183] - BSA and Aside free (Capture) ab275280
- Anti-Mouse-Hif1a antibody [EPR23071-113] - BSA and Aside free (Detector) ab275281

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 15" } ]
style
left-column

Recovery

[ { "sample": "Cell Lysate", "range": "87 - 106 %", "average": "= 96" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325897-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Mouse HIF-1 alpha Capture Antibody", "number":"AB325897-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325897-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325897-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325897-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse HIF-1 alpha Detector Antibody", "number":"AB325897-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB325897-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325897-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325897-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325897-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse HIF-1 alpha Lyophilized Recombinant Protein", "number":"AB325897-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed : 15225651, PubMed : 17981124, PubMed : 22009797). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed : 15225651, PubMed : 17981124, PubMed : 22009797, PubMed : 22100406, PubMed : 37558878). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed : 22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed : 26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).
See full target information Hif1a
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com