JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB216949

Mouse Hsp27 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Hsp27 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Hsp27 with a sensitivity of 72 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Hsp25, Hsp27, Hspb1, Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, Heat shock 27 kDa protein, p25, HSP 25, HSP 27

4 Images
Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)

Interpolated concentrations of native Hsp27 in mouse C2C12 mock-treated, heat-shocked, and untreated cell extracts based on a 1,000 μg/mL extract load.

The concentrations of Hsp27 were measured in duplicate and interpolated from the Hsp27 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hsp27 concentrations were determined to be 680 ng/mg in mock-treated, 1470 ng/mg in heat-shocked, and 700 ng/mg in untreated C2C12 cell extract.

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)

Representative image of Mouse Hsp27 ELISA Kit (ab216949)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Mouse Hsp27 Detector Antibody

- Mouse Hsp27 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Mouse Hsp27 Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CPR2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)

Example of mouse Hsp27 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Hsp27 ELISA Kit (AB216949)

Interpolated concentrations of native Hsp27 in mouse C2C12 cell extract based on a 1,000 μg/mL extract load.

The concentrations of Hsp27 were measured in duplicate and interpolated from the Hsp27 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hsp27 concentration was determined to be 540 ng/mg in C2C12 cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 72 pg/mL

Range

0.23 - 15 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Proteins & Peptides

AB48740

Recombinant Human Hsp27 protein (Tag Free)

Western blot - Recombinant Human Hsp27 protein (Tag Free) (AB48740)

  • 0
  • 0
Primary Antibodies

AB109376

Anti-Hsp27 antibody [EPR5477]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (AB109376)

  • 5
  • 2
Assay Kits

AB133060

HSP70 ELISA Kit

Sandwich ELISA - HSP70 ELISA Kit (AB133060)

  • 4
  • 1
Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse Hsp27 ELISA Kit (ab216949) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hsp27 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Hsp27 with 72 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Heat shock proteins (HSPs) are molecular chaperones that facilitate the proper folding and function of proteins within the cell. Heat shock protein 27 (Hsp27), also known as heat shock protein beta-1 (HspB1) is a 209 amino acid protein encoded by the HSPB1 gene. Hsp27 a member of the sHsp (small heat shock protein) group, whose functions are chaperone activity, thermotolerance, inhibition of apoptosis, and regulation of cellular development and differentiation. Hsp70 member proteins may assist inhibition of apoptosis by acting on the caspase-dependent pathway and against agents such as tumor necrosis factor-alpha, staurosporine, and doxorubicin. Hsp27 can form oligomers depending on the phosphorylation state of the protein and during heat shock. The oligomers are connected to the chaperone activity, while dimers have limited activity. Hsp27 interacts with actin and intermediate filaments, which provides protection to actin filaments from fragmentation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
style
left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "104 - 106 %", "average": "= 105" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab216949-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB216949-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB216949-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Hsp27 Lyophilized Recombinant Protein", "number":"AB216949-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB216949-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB216949-CMP12", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Hsp27 Capture Antibody Lyophilized", "number":"AB216949-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB216949-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB216949-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB216949-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB216949-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Hsp27 Detector Antibody", "number":"AB216949-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB216949-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
style
left-column

Product protocols

style
left-column

Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization (PubMed : 17661394). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (By similarity).
See full target information Hspb1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com