AB100716

Mouse I-TAC ELISA Kit (CXCL11)

Name: Mouse I-TAC ELISA Kit (CXCL11) (ab100716) | Abcam
Brand: Abcam Limited
SKU: AB100716
Price: 780 USD
Availability: InStock

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(2 Publications)

Mouse I-TAC ELISA Kit (CXCL11) is a Sandwich (quantitative) ELISA for the measurement of Mouse I-TAC (CXCL11) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Scyb11, Cxcl11, C-X-C motif chemokine 11, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, I-TAC

2 Images

Sandwich ELISA - Mouse I-TAC ELISA Kit (CXCL11) (AB100716)

sELISA

Unknown

Sandwich ELISA - Mouse I-TAC ELISA Kit (CXCL11) (AB100716)

Representative standard curve using ab100716

sELISA

Unknown

Sandwich ELISA - Mouse I-TAC ELISA Kit (CXCL11) (AB100716)

Representative standard curve using ab100716

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

5.487 - 4000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "67 - 86 %", "average": "= 74.46" }, { "sample": "Serum", "range": "80 - 101 %", "average": "= 91.12" }, { "sample": "Plasma", "range": "68 - 88 %", "average": "= 75.6" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100716-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100716-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100716-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant mouse I-TAC Standard (lyophilized)", "number":"AB100716-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100716-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"I-TAC Microplate (12 x 8 wells)", "number":"AB100716-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse I-TAC", "number":"AB100716-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100716-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100716-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100716-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL11 also known as I-TAC or Interferon-inducible T-cell alpha chemoattractant is a small cytokine belonging to the CXC chemokine family. It weighs around 8 to 11 kilodaltons. CXCL11 predominantly expresses itself in tissues such as the thymus spleen and peripheral blood leukocytes after stimulation with interferons. Its primary role is attracting activated T-cells especially those expressing CXCR3 receptor contributing to immune surveillance and response.

Biological function summary

The chemokine plays an important role in immune system regulation and inflammation response. It attracts and activates leukocytes including T-cells and natural killer cells driving them to sites of inflammation and injury. CXCL11 is not part of a complex but interacts with other chemokines and cytokines to modulate immune responses. Its expression is upregulated in response to IFN-γ working synergistically with other chemokines like CXCL9 and CXCL10 in inflammatory environments.

Pathways

CXCL11 is integral to the chemotaxis signaling pathway. It influences the migration and activation of immune cells by interacting with the CXCR3 receptor. This pathway shares involvement with proteins such as CXCL10 which also binds to CXCR3 enabling precise directionality and regulation of immune cell movement towards inflammation sites. Additionally CXCL11's participation in the inflammatory response pathway connects it to multiple cellular responses necessary for combating infections.

CXCL11 has been linked to autoimmune diseases and cancer. Its elevated expression is associated with conditions like multiple sclerosis where it may contribute to the pathological migration of immune cells into the central nervous system. In cancer CXCL11 can affect tumor microenvironments by recruiting immune cells with cytotoxic potential although this may also facilitate tumor progression in certain contexts. Proteins related to its disease association include CXCR3 and CXCL10 which together modulate various immunological outcomes in these diseases.

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Product protocols

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Target data

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses (By similarity).

See full target information Cxcl11

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 8:7044-7054 PubMed31557409

2019

MACC1 regulates PDL1 expression and tumor immunity through the c-Met/AKT/mTOR pathway in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gangling Tong,Boran Cheng,Jingzhang Li,Xuan Wu,Qiaohong Nong,Lirui He,Xi Li,Laiqing Li,Shubin Wang

Journal of immunology (Baltimore, Md. : 1950) 192:1671-80 PubMed24442435

2014

Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.

Applications

sELISA

Species

Mouse

Erwin Daniel Brenndörfer,Anette Brass,Juliane Karthe,Gustaf Ahlén,Johannes Georg Bode,Matti Sällberg

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse I-TAC ELISA Kit (CXCL11)

Sandwich ELISA - Mouse I-TAC ELISA Kit (CXCL11) (AB100716)

Sandwich ELISA - Mouse I-TAC ELISA Kit (CXCL11) (AB100716)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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