AB100688

Mouse ICAM1 ELISA Kit (CD54)

Name: Mouse ICAM1 ELISA Kit (CD54) (ab100688) | Abcam
Brand: Abcam Limited
SKU: AB100688

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(19 Publications)

Mouse ICAM1 ELISA Kit (CD54) is a Sandwich ELISA for the measurement of Mouse ICAM1 (CD54) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10, Icam1

1 Images

Sandwich ELISA - Mouse ICAM1 ELISA Kit (CD54) (AB100688)

sELISA

Supplier Data

Sandwich ELISA - Mouse ICAM1 ELISA Kit (CD54) (AB100688)

Representative Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

24.69 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's ICAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse ICAM1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 93.54" }, { "sample": "Serum", "range": "84 - 103 %", "average": "= 94.56" }, { "sample": "Plasma", "range": "97 - 127 %", "average": "= 115.2" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100688-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100688-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100688-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ICAM1 Microplate (12 x 8 wells)", "number":"AB100688-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse ICAM1", "number":"AB100688-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100688-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse ICAM1 Standard (lyophilized)", "number":"AB100688-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100688-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100688-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100688-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1.

See full target information Icam1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70023 PubMed40133235

2025

Carbonic anhydrase inhibitors prevent presymptomatic capillary flow disturbances in a model of cerebral amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eugenio Gutiérrez-Jiménez,Peter Mondrup Rasmussen,Irene Klærke Mikkelsen,Sreekanth Kura,Signe K Fruekilde,Brian Hansen,Luca Bordoni,Jasper Carlsen,Johan Palmfeldt,David A Boas,Sava Sakadžić,Sergei Vinogradov,Mirna El Khatib,Jaime Ramos-Cejudo,Boris Wied,Desiree Leduc-Galindo,Elisa Canepa,Adam C Mar,Begona Gamallo-Lana,Silvia Fossati,Leif Østergaard

International journal of molecular sciences 25: PubMed39684207

2024

Endothelial Dysfunction and Impaired Wound Healing Following Radiation Combined Skin Wound Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Bin Lin,Min Zhai,Lisa Hull,Wanchang Cui,Mang Xiao

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23242 PubMed37801065

2023

TIAM2S-positive microglia enhance inflammation and neurotoxicity through soluble ICAM-1-mediated immune priming.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Hsien Chu,Jia-Shing Chen,Ya-Ling Chan,Wei-Jen Lu,Yi-Te Huang,Pin-Cheng Mao,Chun-I Sze,H Sunny Sun

International journal of molecular sciences 24: PubMed37445879

2023

Skin Wound following Irradiation Aggravates Radiation-Induced Brain Injury in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mang Xiao,Xianghong Li,Li Wang,Bin Lin,Min Zhai,Lisa Hull,Alex Zizzo,Wanchang Cui,Juliann G Kiang

Journal of advanced research 54:29-42 PubMed36736696

2023

LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jiang,Yu Song,Mei-Xia Ren,Run-Chao He,Xian-Hui Dong,Xue-Heng Li,Zhi-Feng Lu,Shu Li,Jia Wu,Yan-Rou Bei,Fei Liu,Yan Long,Shao-Guo Wu,Xue-Hui Liu,Li-Mei Wu,Hong-Ling Yang,David G McVey,Xiao-Yan Dai,Shu Ye,Yan-Wei Hu

Frontiers in immunology 13:982045 PubMed36353618

2022

Interaction of RARRES1 with ICAM1 modulates macrophages to suppress the progression of kidney renal clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Geng,Kun Chi,Chao Liu,Zhangning Fu,Xu Wang,Liangliang Meng,Hanfeng Wang,Guangyan Cai,Xiangmei Chen,Quan Hong

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 42:2303-2317 PubMed35999817

2022

Disturbed microcirculation and hyperaemic response in a murine model of systemic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Signe Kirk Fruekilde,Christopher J Bailey,Kate Lykke Lambertsen,Bettina Hjelm Clausen,Jasper Carlsen,Ning-Long Xu,Kim Ryun Drasbek,Eugenio Gutiérrez-Jiménez

Experimental and therapeutic medicine 23:303 PubMed35340877

2022

Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xi Wang,Zhen-Ru Zhao,Ying Bai,Ya-Xing Li,Xiao-Ning Gao,Sen Zhang,Yan-Bin Sun

International journal of molecular sciences 22: PubMed34830227

2021

Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Sapoznikov,Yoav Gal,Yentl Evgy,Moshe Aftalion,Shahaf Katalan,Tamar Sabo,Chanoch Kronman,Reut Falach

Frontiers in immunology 12:745854 PubMed34721415

2021

Endogenous α7 nAChR Agonist SLURP1 Facilitates K1 Crossing the Blood-Brain Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong He,Lei Wang,Liqun Liu,Jie Gao,Beiguo Long,Feng Chi,Tongtong Hu,Yu Wan,Zelong Gong,Li Li,Peilin Zhen,Tiesong Zhang,Hong Cao,Sheng-He Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse ICAM1 ELISA Kit (CD54)

Sandwich ELISA - Mouse ICAM1 ELISA Kit (CD54) (AB100688)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (19)

Product promise