AB205572

Mouse ICAM2 ELISA Kit (CD102)

Recombinant
SimpleStep
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Mouse ICAM2 ELISA Kit (CD102) is a single-wash 90-min Simplestep used to quantify Mouse ICAM2 (CD102) with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD102, Icam-2, Icam2, Intercellular adhesion molecule 2, ICAM-2, Lymphocyte function-associated AG-1 counter-receptor

3 Images

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

sELISA

Supplier Data

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

Example of ICAM2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.

The 1X dilution was 3.13% for the mouse serum, 50.0% for plasma citrate and EDTA, and 25% for plasma heparin. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

Titration of stimulated lung (5 µg/mL LPS (+)), unstimulated lung (-), and unstimulated heart (-) supernatant.

The 1X dilution was 25.0% for the lung supernatants and 6.25% for the heart supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse ICAM2 ELISA Kit (CD102) (ab205572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM2 (CD102) protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ICAM2 (CD102) with 4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 is an integral membrane protein that has 2 immunoglobulin-like domains, and may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.09" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.16" } ]

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Recovery

[ { "sample": "Serum", "range": "96 - 99 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "88 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "107 - 111 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "100 - 108 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "101 - 105 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.

Biological function summary

ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.

Pathways

ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.

ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.

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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

See full target information Icam2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse ICAM2 ELISA Kit (CD102)

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (AB205572)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise