AB252363

Mouse IFN beta ELISA Kit

Recombinant
SimpleStep
What is this?

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(21 Publications)

Mouse IFN beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IFN beta with a sensitivity of 28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

Ifb, Ifnb, Ifnb1, Interferon beta, IFN-beta

4 Images

ELISA - Mouse IFN beta ELISA Kit (AB252363)

ELISA

Supplier Data

ELISA - Mouse IFN beta ELISA Kit (AB252363)

sELISA

Unknown

Sandwich ELISA - Mouse IFN beta ELISA Kit (AB252363)

Interpolated concentrations of native IFN beta in LPS stimulated Raw264.7 cell culture supernatant samples.

The concentrations of IFN beta were measured in duplicates, interpolated from the IFN beta standard curves and corrected for sample dilution. Undiluted samples are as follows : Raw264.7 cell culture supernatant 25%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN beta concentration was determined to be 6,664 pg/mL in Raw264.7 cell culture supernatant. Mouse IFN beta was below the detection point in unstimulated Raw264.7 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse IFN beta ELISA Kit (AB252363)

Example of mouse IFN beta standard curve in Sample Diluent NS.

The IFN beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse IFN beta ELISA Kit (AB252363)

Example of mouse IFN beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 28 pg/mL

Range

39.1 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IFN beta ELISA Kit ab252363 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IFN beta in cell culture media, cell culture supernatant, EDTA plasma, serum. This SimpleStep sensitivity is 28 pg/mL.

How the assay works

Mouse IFN beta SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IFN beta ELISA Kit ab252363 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 100 %", "average": "= 97" }, { "sample": "Serum", "range": "84 - 87 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "81 - 87 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "78 - 98 %", "average": "= 88" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab252363-1x384Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252363-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB252363-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252363-CMP10" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB252363-CMP12" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252363-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB252363-CMP08" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB252363-CMP07" }, { "size":"1 x 600 µL", "name":"10X Mouse IFN beta (Interferon beta 1) Detector Antibody", "number":"AB252363-CMP05" }, { "size":"1 x 600 µL", "name":"10X Mouse IFN beta (Interferon beta 1) Capture Antibody", "number":"AB252363-CMP04" }, { "size":"2 x 1 Vial", "name":"Mouse IFN beta (Interferon beta 1) Lyophilized Recombinant Protein", "number":"AB252363-CMP03" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252363-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252363-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252363-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252363-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252363-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252363-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252363-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IFN beta (Interferon beta 1) Lyophilized Recombinant Protein", "number":"AB252363-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IFN beta (Interferon beta 1) Capture Antibody", "number":"AB252363-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB252363-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IFN beta (Interferon beta 1) Detector Antibody", "number":"AB252363-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252363-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon beta also known as IFN-beta or beta interferon is a type I interferon with a mass of approximately 20 kDa. It originates primarily from fibroblasts and plays an essential role in the immune response. This protein targets and binds to specific cell surface receptors initiating various antiviral states within cells. This action assists in slowing down viral replication and spread. IFN-beta's expression increases in response to viral infections making it an important component in the body's first line of defense against pathogens.

Biological function summary

Interferon beta functions as an important mediator of immune functions regulating the activity of natural killer cells and macrophages. It also boosts antigen presentation to T cells. Interferon beta belongs to the larger family of interferons which includes IFN-alpha and IFN-gamma each having distinct effects but working together to mount an effective immune response. Though it does not form part of a complex it plays a critical standalone role in immune signaling pathways.

Pathways

Interferon beta associates strongly with the JAK-STAT signaling pathway. Upon activation it interacts with receptors to phosphorylate STAT proteins mainly STAT1 and STAT2 which then dimerize and translocate to the nucleus to trigger gene expression. Another important pathway is the antiviral response where interferon beta modulates the expression of hundreds of interferon-stimulated genes. These pathways intricately involve other proteins like IFN-alpha and IFN-gamma which work synergistically to establish an effective antiviral environment.

Interferon beta shows significant implications in multiple sclerosis and hepatitis C infection. It is widely used as a therapeutic agent in managing multiple sclerosis by modulating the immune response to reduce inflammation and slow disease progression. Similarly its role in antiviral defense makes it relevant to hepatitis C infection management. In these contexts IFN-alpha and IFN-gamma are also important as they share similar antiviral and immunomodulatory functions that can complement IFN-beta's actions enhancing the treatment's efficacy.

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Product protocols

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Target data

Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli (PubMed : 10708458, PubMed : 23872679). Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins (By similarity). Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways (PubMed : 23872679). Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor (PubMed : 10708458, PubMed : 14597717). Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons : acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss (PubMed : 26451483). IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (PubMed : 14597717).

See full target information Ifnb1

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Publications (21)

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View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IFN beta ELISA Kit

ELISA - Mouse IFN beta ELISA Kit (AB252363)

Sandwich ELISA - Mouse IFN beta ELISA Kit (AB252363)

Sandwich ELISA - Mouse IFN beta ELISA Kit (AB252363)

Sandwich ELISA - Mouse IFN beta ELISA Kit (AB252363)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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