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AB326435

Mouse IFN beta ELISA Kit, Chemiluminescent

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Mouse IFN beta ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IFN beta with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 8.23 - 18000 pg/ml with a sensitivity of 3.21pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Ifb, Ifnb, Ifnb1, Interferon beta, IFN-beta

4 Images
Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)
  • sELISA

Lab

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)
  • sELISA

Lab

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)

Dynamic range comparison between the Chemiluminescent Mouse IFN beta SimpleStep ELISA, colorimetric Mouse IFN beta SimpleStep ELISA (ab252363), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)
  • sELISA

Lab

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)

Standard curve comparison between the Chemiluminescent Mouse IFN beta SimpleStep ELISA and colorimetric Mouse IFN beta SimpleStep ELISA (ab252363) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)
  • sELISA

Unknown

Sandwich ELISA - Mouse IFN beta ELISA Kit, Chemiluminescent (AB326435)

Interpolated concentrations of native IFN beta in LPS stimulated Raw264.7 cell culture supernatant samples.

The concentrations of IFN beta were measured in duplicates interpolated from the IFN beta standard curves and corrected for sample dilution. Undiluted samples are as follows : Raw264.7 cell culture supernatant 25% The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IFN beta concentration was determined to be 6664 pg/mL in Raw264.7 cell culture supernatant. Mouse IFN beta was below the detection point in unstimulated Raw264.7 supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Cell culture media

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.21 pg/mL

Range

8.23 - 18000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IFN beta ELISA Kit, Chemiluminescent (ab326435) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IFN beta in Cell culture supernatant, Serum, EDTA Plasma, Cell culture media with a sensitivity of 3.21 pg/mL.

How the assay works

Mouse IFN beta ELISA Kit, Chemiluminescent (ab326435) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IFN beta (ab326435) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-mouse Interferon beta 1 antibody [EPR22186-226] - BSA and Aside free (Capture) ab259661
- Anti-mouse Interferon beta 1 antibody [EPR22186-242] - BSA and Aside free (Detector) ab259662
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 100 %", "average": "= 97" }, { "sample": "Serum", "range": "84 - 87 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "81 - 87 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "78 - 98 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326435-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326435-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326435-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IFN beta Detector Antibody", "number":"AB326435-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IFN beta Lyophilized Recombinant Protein", "number":"AB326435-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IFN beta Capture Antibody", "number":"AB326435-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326435-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326435-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326435-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB326435-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326435-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli (PubMed : 10708458, PubMed : 23872679). Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins (By similarity). Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways (PubMed : 23872679). Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor (PubMed : 10708458, PubMed : 14597717). Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons : acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss (PubMed : 26451483). IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (PubMed : 14597717).
See full target information Ifnb1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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