Mouse IFN gamma ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IFN gamma with a sensitivity of 1.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
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- Frontiers in immunology 14:11786382023Netrin-1 controls inflammation in response to ischemic stroke through altering microglia phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaosheng Yang,Yang Liu,Weijie Zhong,Yi Li,Wenchuan ZhangPubMed 37388740
- Frontiers in immunology 14:11967042023Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Huang,Wei Gao,Xin Zhong,Hongxian Wu,You Zhou,Yuanji Ma,Juying Qian,Junbo GePubMed 37215106
- Frontiers in immunology 14:11703882023Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyan Fan,Hongming Xu,Chenling Shen,Jia Fang,Xiaoyan LiPubMed 37122744
- Antioxidants (Basel, Switzerland) 12:2023Antioxidant and Immune Stimulating Effects of Skin in the RAW 264.7 Cells and in the C57BL/6 Mouse Immunosuppressed by Cyclophosphamide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Su Kim,Eun-Byeol Lee,Ji-Hye Choi,Jieun Jung,Un-Yul Jeong,Ui-Jin Bae,Hwan-Hee Jang,Shin-Young Park,Youn-Soo Cha,Sung-Hyen LeePubMed 37107267
- Frontiers in immunology 14:11656022023Loss of NLRP3 reduces oxidative stress and polarizes intratumor macrophages to attenuate immune attack on endometrial cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolu Zhu,Yanli Xu,Juan Wang,Zhuowei Xue,Tian Qiu,Jing ChenPubMed 37077909
- Discover. Oncology 14:322023Radiotherapy combined with anti-CEACAM1 immunotherapy to induce survival advantage in glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhu Li,Yi Chen,Yimin Fan,Hongqin Wang,Wei Mu,Xiaodong LiuPubMed 36928507
- Frontiers in immunology 13:10643032022Persistent expression of NLRP3 in spinal microglia promotes development of lumbar disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Wang,Jing ZhangPubMed 36505450
- Frontiers in immunology 13:10577462022NLRP3+ macrophages aggravate inflammatory cystitis in diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYubing Peng,Yan GaoPubMed 36405726
- American journal of translational research 14:6874-68882022IFN-γ promotes the development of systemic lupus erythematosus through the IFNGR1/2-PSTAT1-TBX21 signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Bailing TianPubMed 36398225
- Oxidative medicine and cellular longevity 2022:86192752022Delicaflavone Represses Lung Cancer Growth by Activating Antitumor Immune Response through N6-Methyladenosine Transferases and Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuewen Wang,Dafen Xu,Bing Chen,Dandan Huang,Zhijun Li,Yuxia Sui,Fei Lin,Hong Yao,Huang Yuan Li,Xinhua LinPubMed 35979397
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma, Citrate plasma, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.813 - 500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.9 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:11585387, PubMed:8456301). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (PubMed:20535209, PubMed:25078851).
Alternative names
Interferon gamma, IFN-gamma, Ifng
What's included?
Recommended products
Mouse IFN gamma ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IFN gamma with a sensitivity of 1.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma, Citrate plasma, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.813 - 500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.9 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 1.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106 | Range 101 - 114 % |
Sample type Serum | Average % = 95 | Range 85 - 102 % |
Sample type EDTA Plasma | Average % = 103 | Range 102 - 103 % |
Sample type Heparin Plasma | Average % = 93 | Range 89 - 95 % |
Sample type Citrate plasma | Average % = 95 | Range 93 - 98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse IFN gamma ELISA kit (ab282874) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IFN gamma protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IFN gamma with 1.9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IFN gamma (Interferon-gamma), is an important immunoregulatory cytokine in addition of having antiviral activity. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. IFN gamma is produced by lymphocytes activated by specific antigens or mitogens.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
- Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
- Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
- Associated diseases and disorders
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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6 product images
Sandwich ELISA - Mouse IFN gamma ELISA Kit (ab282874)
Human IFN gamma competitor curve comparison.
Standard Curve comparison between Mouse IFN gamma SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Mouse IFN gamma ELISA Kit (ab282874)
Example of mouse IFN gamma standard curve in Sample Diluent NS.
The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IFN gamma ELISA Kit (ab282874)
Interpolated concentrations of native IFN gamma in CD-1 mouse serum and plasma samples.
The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 20.0 pg/mL in neat serum, 20.4 pg/mL in neat plasma (citrate) 37.6 pg/mL in neat plasma (EDTA) and 23.5 pg/mL in neat plasma (heparin).
Sandwich ELISA - Mouse IFN gamma ELISA Kit (ab282874)
Interpolated concentrations of native IFN gamma in C57BL/6 and BALB/C mouse serum samples.
The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: C57BL/6 serum 100% and BALB/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 22.1 pg/mL in neat C57BL/6 serum and 17.4 pg/mL in neat BALB/C serum.
Sandwich ELISA - Mouse IFN gamma ELISA Kit (ab282874)
Interpolated concentrations of native IFN gamma in mouse spleen supernatant samples treated with or without 5 µg/mL LPS for 6 days.
The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS treated spleen supernatant 15% and mock treated spleen supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 2,347 pg/mL in LPS treated neat spleen supernatant and 51 pg/mL in mock treated neat spleen supernatant.
Sandwich ELISA - Mouse IFN gamma ELISA Kit (ab282874)
Neat serum samples from five individual healthy CD-1, BALB/C and wild type C57BL/6 mouse strain donors and two individual C57BL/6 IFN gamma knockout mouse donors were measured in duplicate.
Interpolated values of individual sera are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 25 pg/mL with a range of undetectable – 63 pg/mL in CD-1 serum, 21 pg/mL with a range of undetectable – 42 pg/mL in BALB/C serum, 168 pg/mL with a range of 85 – 274 pg/mL in wild type C57BL/6 serum and undetectable in IFN gamma knockout C57BL/6 serum.
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