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AB282874

Mouse IFN gamma ELISA Kit

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(19 Publications)

Mouse IFN gamma ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IFN gamma with a sensitivity of 1.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Interferon gamma, IFN-gamma, Ifng

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ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • ELISA

Supplier Data

ELISA - Mouse IFN gamma ELISA Kit (AB282874)
Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Interpolated concentrations of native IFN gamma in mouse spleen supernatant samples treated with or without 5 µg/mL LPS for 6 days.

The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : LPS treated spleen supernatant 15% and mock treated spleen supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 2,347 pg/mL in LPS treated neat spleen supernatant and 51 pg/mL in mock treated neat spleen supernatant.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Interpolated concentrations of native IFN gamma in CD-1 mouse serum and plasma samples.

The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 20.0 pg/mL in neat serum, 20.4 pg/mL in neat plasma (citrate) 37.6 pg/mL in neat plasma (EDTA) and 23.5 pg/mL in neat plasma (heparin).

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Human IFN gamma competitor curve comparison.

Standard Curve comparison between Mouse IFN gamma SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Neat serum samples from five individual healthy CD-1, BALB/C and wild type C57BL/6 mouse strain donors and two individual C57BL/6 IFN gamma knockout mouse donors were measured in duplicate.

Interpolated values of individual sera are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 25 pg/mL with a range of undetectable – 63 pg/mL in CD-1 serum, 21 pg/mL with a range of undetectable – 42 pg/mL in BALB/C serum, 168 pg/mL with a range of 85 – 274 pg/mL in wild type C57BL/6 serum and undetectable in IFN gamma knockout C57BL/6 serum.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Example of mouse IFN gamma standard curve in Sample Diluent NS.

The IFN gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Interpolated concentrations of native IFN gamma in C57BL/6 and BALB/C mouse serum samples.

The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : C57BL/6 serum 100% and BALB/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 22.1 pg/mL in neat C57BL/6 serum and 17.4 pg/mL in neat BALB/C serum.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Lab

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Example of mouse IFN gamma standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Lab

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Interpolated concentration of native IFN gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 15% LPS treated spleen supernatant (5 μg/mL, 6 days). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)
  • sELISA

Lab

Sandwich ELISA - Mouse IFN gamma ELISA Kit (AB282874)

Example of mouse IFN gamma standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma, Citrate plasma, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.9 pg/mL

Range

7.813 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IFN gamma ELISA Kit ab282874 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IFN gamma in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 1.9 pg/mL.

How the assay works

Mouse IFN gamma SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IFN gamma ELISA Kit ab282874 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Interferon gamma antibody [EPR20701-81] - BSA and Azide free (Capture) ab281086
- Anti-Interferon gamma antibody [EPR20701-26] - BSA and Azide free (Detector) ab281086

IFN gamma (Interferon-gamma), is an important immunoregulatory cytokine in addition of having antiviral activity. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. IFN gamma is produced by lymphocytes activated by specific antigens or mitogens.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 114 %", "average": "= 106" }, { "sample": "Serum", "range": "85 - 102 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "89 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "93 - 98 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of na&luml;ve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 11585387, PubMed : 8456301). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (PubMed : 20535209, PubMed : 25078851).
See full target information Ifng
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 13: PubMed40266088

2025

Immunogenicity of Rabies Virus G-Protein mRNA Formulated with Muscle-Targeting Lipid Nanoparticles in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Li,Huarong Bai,Xueliang Yu,Qiang Liu,Rongkuan Hu

Nature communications 16:2028 PubMed40016240

2025

Aspirin-responsive gene switch regulating therapeutic protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbo Huang,Ana Palma Teixeira,Ting Gao,Shuai Xue,Mingqi Xie,Martin Fussenegger

Vaccines 13: PubMed40006715

2025

Enhanced and Prolonged Immunogenicity in Mice of Thermally Stabilized Fatty Acid-Conjugated Vaccine Antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Mi Kim,Yeon-Ho Kim,Hai V Ngo,Hy D Nguyen,Chulhun Park,Beom-Jin Lee

Biomedicines 12: PubMed39062070

2024

Newcastle Disease Virus Virotherapy: Unveiling Oncolytic Efficacy and Immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kawther A Zaher,Jehan S Alrahimi,Fatemah S Basingab,Alia M Aldahlawi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309411 PubMed38741284

2024

Ultrashort-Peptide-Responsive Gene Switches for Regulation of Therapeutic Protein Expression in Mammalian Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbo Huang,Shuai Xue,Yu-Qing Xie,Ana Palma Teixeira,Martin Fussenegger

Molecular biology reports 51:213 PubMed38280039

2024

Desmosterol-driven atypical macrophage polarization regulates podocyte dynamics in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Huiying Qi

Cellular and molecular life sciences : CMLS 81:32 PubMed38214780

2024

Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Jin,Meixin Shi,Jing Feng,Zhengwei Zhang,Bingbing Zhao,Qingyu Li,Ligen Yu,Zhaoyang Lu

Frontiers in immunology 14:1178638 PubMed37388740

2023

Netrin-1 controls inflammation in response to ischemic stroke through altering microglia phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosheng Yang,Yang Liu,Weijie Zhong,Yi Li,Wenchuan Zhang

Frontiers in immunology 14:1196704 PubMed37215106

2023

Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Huang,Wei Gao,Xin Zhong,Hongxian Wu,You Zhou,Yuanji Ma,Juying Qian,Junbo Ge

Frontiers in immunology 14:1170388 PubMed37122744

2023

Nrf2 orchestrates transition from acute to chronic otitis media through inflammatory macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Fan,Hongming Xu,Chenling Shen,Jia Fang,Xiaoyan Li
View all publications
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