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AB267580

Mouse IFNGR1 ELISA Kit

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(1 Publication)

Mouse IFNGR1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IFNGR1 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD119, Ifngr, Ifngr1, Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha

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Sandwich ELISA - Mouse IFNGR1 ELISA Kit (AB267580)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IFNGR1 ELISA Kit (AB267580)

Mouse IFNGR1 ELISA kit (ab267580) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.16 ng/mL

Range

0.164 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IFNGR1 ELISA Kit is designed for the quantitative determination of IFNGR1 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for mouse IFNGR1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IFNGR1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IFNGR1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFNGR1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects mouse IFN-gamma R1. No cross-reactivity with mouse IFNalpha/beta R1, mouse IFN-gamma R2, human IFN-gamma R1 is observed.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "102 - 115 %", "average": "= 108" }, { "sample": "Plasma", "range": "67 - 128 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "79 - 93 %", "average": "= 87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267580-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267580-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IFNGR1 Standard (Lyophilized)", "number":"AB267580-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267580-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse IFNGR1 coated Microplate (12 x 8 wells)", "number":"AB267580-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267580-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267580-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse IFNGR1 Detection Antibody", "number":"AB267580-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB267580-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IFNGR1 protein also known as Interferon Gamma Receptor 1 is an integral component of the cell surface receptor for interferon-gamma (IFN-γ). It possesses a mass of approximately 53 kDa. IFNGR1 is expressed in various cell types including immune cells such as T cells and macrophages and is also present in other cell lines like HEK 293. This receptor binds to its specific ligand IFN-γ initiating a series of intracellular events that are essential for immune response modulation.
Biological function summary

IFNGR1 functions as part of the interferon-gamma receptor complex working alongside the IFNGR2 subunit. This interaction with IFNGR2 is important for the receptor to properly transmit signals inside the cell. The binding of interferon-gamma to this receptor complex activates the JAK-STAT signaling pathway promoting the transcription of genes that enhance the antimicrobial activity of immune cells and regulate cellular immunity.

Pathways

The IFNGR1 protein plays an important role in the JAK-STAT signaling pathway which is critical for mediating responses to interferon-gamma. Upon activation by IFN-γ IFNGR1 recruits JAK kinases leading to the phosphorylation of STAT1 a significant transcription factor. STAT1 then dimerizes and translocates to the nucleus where it induces the expression of genes involved in immune defense and inflammation. This process is vital for the body's ability to handle infections and other immunological challenges.

IFNGR1 is associated with susceptibility to infections and certain immune-related disorders. Mutations or deficiencies in IFNGR1 can lead to Mendelian Susceptibility to Mycobacterial Disease (MSMD) where individuals show increased vulnerability to mycobacterial infections. Additionally improper signaling through IFNGR1 is linked to chronic granulomatous disease which can involve defective functioning of the NADPH oxidase complex. Understanding IFNGR1's function and interactions is important for exploring new therapeutic strategies for these disorders.
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Product protocols

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Target data

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed : 20926559, PubMed : 27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed : 2137461, PubMed : 2530216, PubMed : 2530582, PubMed : 2531896, PubMed : 2532365). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed : 19889125). STAT3 can also be activated in a similar manner although activation seems weaker (PubMed : 15284232). IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (PubMed : 15522878).
See full target information Ifngr1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 13:4461-4476 PubMed37969726

2023

A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Li,Xiangqing Ding,Xianxian Wu,Longfei Ding,Yuhui Yang,Xiaoliang Jiang,Xing Liu,Xu Zhang,Jianrong Su,Jianqing Xu,Zhiwei Yang
View all publications
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