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AB157717

Mouse IgA ELISA Kit

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(8 Publications)

Mouse IgA ELISA Kit is a sandwich ELISA designed to quantify Mouse IgA with a sensitivity of 1.4102 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 12.5 - 400 ng/mL
- Cited in over 10 publications

View Alternative Names

Ig alpha chain C region

3 Images
Sandwich ELISA - Mouse IgA ELISA Kit (AB157717)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IgA ELISA Kit (AB157717)

Standard curve : mean of duplicates (+/-SD) with background readings subtracted

Sandwich ELISA - Mouse IgA ELISA Kit (AB157717)
  • sELISA

Lab

Sandwich ELISA - Mouse IgA ELISA Kit (AB157717)

Mouse IgA measured in mouse tissue lysates showing quantity (pg) per microgram of protein

Sandwich ELISA - Mouse IgA ELISA Kit (AB157717)
  • sELISA

Lab

Sandwich ELISA - Mouse IgA ELISA Kit (AB157717)

Mouse IgA measured in biologicals showing quantity (ng) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.4102 ng/mL

Range

12.5 - 400 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse IgA ELISA Kit ab157717 is a sandwich ELISA to measure Mouse IgA in serum, plasma, other biological fluids with a sensitivity of 1.4102 ng/ml.

How the assay works

In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse IgA ELISA Kit ab157717 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157717-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157717-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse IgA Calibrator (lyophilized)", "number":"AB157717-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157717-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157717-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Mouse IgA ELISA Microplate", "number":"AB157717-CMP05", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-mouse IgA antibody", "number":"AB157717-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157717-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.
Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.
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Product protocols

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Target data

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
See full target information
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0273604 PubMed37556447

2023

Identification of novel lactic acid bacteria with enhanced protective effects against influenza virus.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Sugimoto,Tomoe Numaguchi,Ryota Chihama,Yuto Takenaka,Yuuki Sato

Nature materials 22:903-912 PubMed36759564

2023

An inhaled bioadhesive hydrogel to shield non-human primates from SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Mei,Junlang Li,Zhenzhen Wang,Dashuai Zhu,Ke Huang,Shiqi Hu,Kristen D Popowski,Ke Cheng

International journal of molecular sciences 23: PubMed36361614

2022

Potential Antigenic Candidates for the Development of Peptide-Based Vaccines to Induce Immunization against Infection in BALB/c Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Doaa M AlEraky,Hatem M Abuohashish,Amr S Bugshan,Maha M Abdelsalam,Hussain A AlHawaj,Taleb T AlKhamis,Fatimah A AlDossary,Nabras M Alrayes,Yasser M Ragab,Zeinab AbdelKhalek,Omneya M Helmy,Mohammed A Ramadan

Journal of immunology research 2022:7200379 PubMed35465352

2022

Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Dang Sheng,Sui Xin,Wu Dongxing,Wen Shubo,Song Yang,Chen Zeliang,Zhai Jingbo

Journal of lipid research 63:100153 PubMed34808192

2021

Oxidized phospholipids cause changes in jejunum mucus that induce dysbiosis and systemic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Pallavi Mukherjee,Arnab Chattopadhyay,Victor Grijalva,Nasrin Dorreh,Venu Lagishetty,Jonathan P Jacobs,Bethan L Clifford,Thomas Vallim,Julia J Mack,Mohamad Navab,Srinivasa T Reddy,Alan M Fogelman

Acta pharmaceutica (Zagreb, Croatia) 70:483-498 PubMed32412434

2020

Synthesis and evaluation of bisulfate/mesylate-conjugated chlortetracycline with high solubility and bioavailability.

Applications

Unspecified application

Species

Unspecified reactive species

Deng Liu,Peixing Chen,Xu Yang,Jianhua Wang

Nature 577:543-548 PubMed31915378

2020

Microbiota-targeted maternal antibodies protect neonates from enteric infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zheng,Wenjing Zhao,Meng Wu,Xinyang Song,Florence Caro,Ximei Sun,Francesca Gazzaniga,Giuseppe Stefanetti,Sungwhan Oh,John J Mekalanos,Dennis L Kasper

Journal of extracellular vesicles 8:1632100 PubMed31275534

2019

Bioengineering commensal bacteria-derived outer membrane vesicles for delivery of biologics to the gastrointestinal and respiratory tract.

Applications

Unspecified application

Species

Unspecified reactive species

Ana L Carvalho,Sonia Fonseca,Ariadna Miquel-Clopés,Kathryn Cross,Khoon-S Kok,Udo Wegmann,Katherine Gil-Cordoso,Eleanor G Bentley,Sanaria H M Al Katy,Janine L Coombes,Anja Kipar,Regis Stentz,James P Stewart,Simon R Carding
View all publications
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