Mouse IgE ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Mouse IgE with a sensitivity of 7.263 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Citrate plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Rat
- Range
- 25 - 1600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.263 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Alternative names
Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, IGHE
What's included?
Recommended products
Mouse IgE ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Mouse IgE with a sensitivity of 7.263 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Citrate plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse, Rat
- Range
- 25 - 1600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.263 pg/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 6.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 0.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 91 - 107 % |
Sample type Citrate plasma | Average % = 102 | Range 97 - 105 % |
Sample type EDTA Plasma | Average % = 104 | Range 97 - 111 % |
Sample type Heparin Plasma | Average % = 99 | Range 84 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse IgE SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgE protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma. Quantitate Mouse IgE with 7.263 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.
- Biological function summary
The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.
- Pathways
IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.
- Associated diseases and disorders
IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.
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7 product images
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Example of mouse IgE standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Interpolated concentrations of mouse IgE in mouse serum and plasma (heparin). Interpolated concentration of native IgE was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse serum 1:4,000 and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Interpolated concentrations of IgE in rat serum, plasma (EDTA), plasma (citrate), and plasma (heparin). Interpolated concentration of native IgE was measured in duplicate at different sample concentrations. Undiluted samples are as follows: rat serum 5%, plasma (EDTA) 6.25%, plasma citrate) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Interpolated concentrations of mouse IgE in mouse plasma (citrate) and plasma (EDTA). Interpolated concentration of native IgE was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse plasma (citrate) 1:200 and plasma (EDTA) 1:200. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Interpolated concentrations of IgE in normal mouse serum donors. Serum of six individual healthy female mouse donors was measured in duplicate. Donors #1-2 were C57BL/6, donors #3-4 were CD-1, and donors #5-6 were BALB/c. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgE concentration was determined to be 52.1 ng/mL with a range of 28.8 - 78.6 ng/mL.
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Example of mouse IgE standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IgE ELISA Kit- Extracellular (ab317773)
Interpolated concentrations of mouse IgE in mouse plasma (EDTA) in 96-well vs. 384-well plates. Interpolated concentration of native IgE was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:200 plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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