AB317774

Mouse IgE ELISA Kit- Intracellular

SimpleStep
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Mouse IgE ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Mouse IgE with a sensitivity of 8.857 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, IGHE

3 Images

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

sELISA

Supplier Data

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

Interpolated concentrations of mouse IgE in mouse spleen extract. Interpolated concentration of native IgE was measured in duplicate at different sample concentrations. Undiluted samples are 500 ug/mL mouse spleen extract and mouse skeletal muscle extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. No IgE was detected in skeletal muscle extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

Example of mouse IgE standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

Example of mouse IgE standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 8.857 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IgE SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgE protein in Tissue extracts . Quantitate Mouse IgE with 8.857 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.7" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "103 - 109 %", "average": "= 106" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317774-10x96Tests", "assetComponentDetails": [ { "size":"10 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB317774-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317774-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317774-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317774-CMP12" }, { "size":"20 x 1 Vial", "name":"Mouse IgE Lyophilized Recombinant Protein", "number":"AB317774-CMP09" }, { "size":"10 x 600 µL", "name":"10X Mouse IgE Capture Antibody", "number":"AB317774-CMP01" }, { "size":"10 x 600 µL", "name":"10X Mouse IgE Detector Antibody", "number":"AB317774-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317774-CMP05" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317774-CMP11" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317774-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317774-1x384Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB317774-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317774-CMP04" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317774-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317774-CMP12" }, { "size":"2 x 1 Vial", "name":"Mouse IgE Lyophilized Recombinant Protein", "number":"AB317774-CMP09" }, { "size":"1 x 600 µL", "name":"10X Mouse IgE Capture Antibody", "number":"AB317774-CMP01" }, { "size":"1 x 600 µL", "name":"10X Mouse IgE Detector Antibody", "number":"AB317774-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317774-CMP07" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317774-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317774-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317774-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB317774-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317774-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317774-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317774-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317774-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IgE Lyophilized Recombinant Protein", "number":"AB317774-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IgE Capture Antibody", "number":"AB317774-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IgE Detector Antibody", "number":"AB317774-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317774-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB317774-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317774-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.

Biological function summary

The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.

Pathways

IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.

IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.

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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.

See full target information IGHE

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IgE ELISA Kit- Intracellular

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

Sandwich ELISA - Mouse IgE ELISA Kit- Intracellular (AB317774)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Product promise