Mouse IGFBP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IGFBP1 with a sensitivity of 15.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 34.38 - 2200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 15.55 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner. Plays also a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif. Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway (By similarity). Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation (By similarity).
Alternative names
Igfbp-1, Igfbp1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1
What's included?
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Mouse IGFBP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IGFBP1 with a sensitivity of 15.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 34.38 - 2200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 15.55 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99 | Range 92 - 107 % |
Sample type Serum | Average % = 111 | Range 105 - 119 % |
Sample type EDTA Plasma | Average % = 103 | Range 99 - 108 % |
Sample type Heparin Plasma | Average % = 110 | Range 94 - 122 % |
Sample type Citrate plasma | Average % = 99 | Range 97 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse IGFBP1 ELISA kit (ab272465) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IGFBP1 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP1 with 15.55 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Insulin-like growth factor-binding protein 1 (IGFBP1) is an IGF binding protein involved in prolonging its half-life and has both inhibitory and stimulatory effects on IGFs. IGFBP1 can also alter the interaction of IGFs with their receptors. IGFBP1 is additionally involved in cell migration.
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3 product images
Sandwich ELISA - Mouse IGFBP1 ELISA Kit (ab272465)
Example of mouse IGFBP1 standard curve in Sample Diluent NS.
The IGFBP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse IGFBP1 ELISA Kit (ab272465)
Sandwich ELISA - Mouse IGFBP1 ELISA Kit (ab272465)
Interpolated concentrations of native IGFBP1 in mouse liver tissue supernatant samples.
The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 4.69 ng/mL in liver supernatant.
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