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AB100691

Mouse IGFBP2 ELISA Kit

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(1 Publication)

Mouse IGFBP2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IGFBP2 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Igfbp-2, Igfbp2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, mIGFBP-2

1 Images
Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB100691)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB100691)

Typical Standard Curve

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 0.6 ng/mL

Range

0.823 - 600 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IGFBP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse IGFBP2 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 122 %", "average": "= 112.5" }, { "sample": "Serum", "range": "72 - 90 %", "average": "= 81.45" }, { "sample": "Plasma", "range": "73 - 92 %", "average": "= 80.22" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100691-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100691-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100691-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IGFBP2 Standard (lyophilized)", "number":"AB100691-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100691-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IGFBP2", "number":"AB100691-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100691-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100691-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100691-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IGFBP2 Microplate (12 x 8 wells)", "number":"AB100691-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 18276763). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed : 26773517). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury.
See full target information Igfbp2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrition, metabolism, and cardiovascular diseases 24:57-64 PubMed23831006

2013

Hepatic molecular responses to Bifidobacterium pseudocatenulatum CECT 7765 in a mouse model of diet-induced obesity.

Applications

Unspecified application

Species

Unspecified reactive species

A Moya-Pérez,M Romo-Vaquero,F Tomás-Barberán,Y Sanz,M-T García-Conesa
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