AB207615

Mouse IGFBP2 ELISA Kit

Recombinant
SimpleStep
What is this?

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(6 Publications)

Mouse IGFBP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IGFBP2 with a sensitivity of 103.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Igfbp-2, Igfbp2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, mIGFBP-2

3 Images

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

Example of mouse IGFBP2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

Interpolated concentrations of native IGFBP2 in mouse serum and plasma samples.

The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1.25%, plasma (citrate) 1%, plasma (heparin) 1% and plasma (EDTA) 1 : 160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 492.8 ng/mL in serum, 325.6 ng/mL in plasma (citrate), 428.3 ng/mL in plasma (heparin) and 1247.2 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

Rat IGFBP2 is detectable using this assay.

The concentrations of IGFBP2 in rat serum were measured in duplicates, interpolated from the mouse IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are serum 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 357.6 ng/mL in rat serum.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 103.6 pg/mL

Range

179.68 - 11500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse IGFBP2 ELISA Kit (ab207615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP2 with 103.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

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Recovery

[ { "sample": "Serum", "range": "92 - 102 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 111 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "77 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "95 - 107 %", "average": "= 101" }, { "sample": "Tissue Culture Media", "range": "94 - 96 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 18276763). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed : 26773517). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury.

See full target information Igfbp2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Brain sciences 13: PubMed37891752

2023

Molecular Study of the Protective Effect of a Low-Carbohydrate, High-Fat Diet against Brain Insulin Resistance in an Animal Model of Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulhadi Bima,Basmah Eldakhakhny,Aliaa A Alamoudi,Zuhier Awan,Abrar Alnami,Salwa Mohamed Abo-Elkhair,Hussein Sakr,Fatma Mohamed Ghoneim,Ayman Elsamanoudy

Molecular therapy : the journal of the American Society of Gene Therapy 29:2514-2534 PubMed33940157

2021

Multi-omics comparisons of different forms of centronuclear myopathies and the effects of several therapeutic strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Djeddi,David Reiss,Alexia Menuet,Sébastien Freismuth,Juliana de Carvalho Neves,Sarah Djerroud,Xènia Massana-Muñoz,Anne-Sophie Sosson,Christine Kretz,Wolfgang Raffelsberger,Céline Keime,Olivier M Dorchies,Julie Thompson,Jocelyn Laporte

Scientific reports 10:12915 PubMed32737396

2020

A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Magdalen Greco,Stefano Garetto,Emilie Montellier,Yu Liu,Siwei Chen,Pierre Baldi,Paolo Sassone-Corsi,Jacopo Lucci

Journal of the Endocrine Society 4:bvz006 PubMed32190801

2020

IGFBP-1 in Cardiometabolic Pathophysiology-Insights From Loss-of-Function and Gain-of-Function Studies in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie J Haywood,Thomas A Slater,Michael Drozd,Nele Warmke,Connor Matthews,Paul A Cordell,Jessica Smith,Jethro Rainford,Harneet Cheema,Caitlyn Maher,Katherine I Bridge,Nadira Y Yuldasheva,Richard M Cubbon,Mark T Kearney,Stephen B Wheatcroft

American journal of physiology. Endocrinology and 318:E723-E735 PubMed31910027

2020

Downregulation of microRNA-873 attenuates insulin resistance and myocardial injury in rats with gestational diabetes mellitus by upregulating IGFBP2.

Applications

Unspecified application

Species

Unspecified reactive species

Na Han,Hai-Yan Fang,Jie-Xuan Jiang,Qian Xu

BMC gastroenterology 19:96 PubMed31221091

2019

Linear growth failure induced by systemic inflammation inhibiting IGF-1/IGFBP axis in rats with asymptomatic colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyang Sheng,Xueqing Sun,Feng Li,Junli Wang,Jingqiu Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IGFBP2 ELISA Kit

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

Sandwich ELISA - Mouse IGFBP2 ELISA Kit (AB207615)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

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