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AB100692

Mouse IGFBP3 ELISA Kit

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(3 Publications)

Mouse IGFBP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IGFBP3 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Igfbp-3, Igfbp3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

3 Images
Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB100692)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IGFBP3 ELISA Kit (AB100692)

Representative Standard Curve using ab100692

ELISA - Mouse IGFBP3 ELISA Kit (AB100692)
  • ELISA

Lab

ELISA - Mouse IGFBP3 ELISA Kit (AB100692)

IGFBP3 in mouse tissue lysates extracted with RIPA buffer. Data from kidney lysates diluted 1/50-1/250 (protein concentration 0.25-0.05 mg x mL-1) and lung diluted 1/10 (0.75 mg x mL-1) (duplicates +/- SD).

ELISA - Mouse IGFBP3 ELISA Kit (AB100692)
  • ELISA

Lab

ELISA - Mouse IGFBP3 ELISA Kit (AB100692)

IGFBP3 in mouse biological fluids (duplicates +/- SD). Plasma and serum tested in the dilution range of 1/50-1/250, urine tested undiluted, rat and human samples (serum and plasma) were below level of detection at 1/10 dilution.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 70 pg/mL

Range

54.87 - 40000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IGFBP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP3 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse IGFBP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 112 %", "average": "= 105.5" }, { "sample": "Serum", "range": "73 - 94 %", "average": "= 85.83" }, { "sample": "Plasma", "range": "71 - 84 %", "average": "= 76.81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100692-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100692-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100692-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IGFBP3 Microplate (12 x 8 wells)", "number":"AB100692-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100692-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100692-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100692-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100692-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IGFBP3 Standard (lyophilized)", "number":"AB100692-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse IGFBP3", "number":"AB100692-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.
See full target information Igfbp3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed32078587

2020

Osteoclast-derived IGF1 is required for pagetic lesion formation in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuaki Miyagawa,Yasuhisa Ohata,Jesus Delgado-Calle,Jumpei Teramachi,Hua Zhou,David D Dempster,Mark A Subler,Jolene J Windle,John M Chirgwin,G David Roodman,Noriyoshi Kurihara

Scientific reports 6:31853 PubMed27558773

2016

Space radiation exposure persistently increased leptin and IGF1 in serum and activated leptin-IGF1 signaling axis in mouse intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Shubhankar Suman,Santosh Kumar,Albert J Fornace,Kamal Datta

International journal of biological sciences 11:274-83 PubMed25678846

2015

Protracted upregulation of leptin and IGF1 is associated with activation of PI3K/Akt and JAK2 pathway in mouse intestine after ionizing radiation exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Shubhankar Suman,Bhaskar V S Kallakury,Albert J Fornace,Kamal Datta
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com